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DNA Cloning: A Hands-on Approach

Authors: Choi, Seok-Yong, Ro, Hyunju, Yi, Hankuil

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  • Clear step-by-step description of the full range of methods
  • Advice on fundamentals and prerequisites
  • Guidance on avoidance of errors
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eBook $129.00
price for Mexico (gross)
  • ISBN 978-94-024-1662-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $169.99
price for Mexico
  • ISBN 978-94-024-1660-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail. 

About the authors

Seok-Yong Choi, Department of Biomedical Sciences, Chonnam National University College of Medicine, Gwangju, Republic of Korea
Hyunju Ro, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
Hankuil Yi, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea

Table of contents (7 chapters)

Table of contents (7 chapters)

Buy this book

eBook $129.00
price for Mexico (gross)
  • ISBN 978-94-024-1662-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for Mexico
  • ISBN 978-94-024-1660-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Cloning: A Hands-on Approach
Authors
Copyright
2019
Publisher
Springer Netherlands
Copyright Holder
Springer Nature B.V.
eBook ISBN
978-94-024-1662-6
DOI
10.1007/978-94-024-1662-6
Hardcover ISBN
978-94-024-1660-2
Edition Number
1
Number of Pages
XIII, 131
Number of Illustrations
23 b/w illustrations, 70 illustrations in colour
Topics