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Imaging, Manipulation and Optogenetics in Zebrafish

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  • © 2018

Overview

Authors:
  1. Itia Amandine Favre‐Bulle

    1. School of Mathematics and Physics, The University of Queensland, Brisbane, Australia

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  • Nominated as an outstanding Ph.D thesis by the University of Queensland, Sydney, Australia
  • Develops and combines novel optical methods for micromanipulation of neuronal system
  • Describes an intensively pursued research area that promises important advances in basic neuroscience

Part of the book series: Springer Theses (Springer Theses)

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Table of contents (7 chapters)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Introduction

    • Itia Amandine Favre-Bulle
    Pages 1-7

  3. Light Scattering in Brain Tissue Using Monte Carlo Method

    • Itia Amandine Favre-Bulle
    Pages 9-20

  4. Scattering in Zebrafish Brain for Optogenetics

    • Itia Amandine Favre-Bulle
    Pages 21-31

  5. Optical Systems to Decode Brain Activity

    • Itia Amandine Favre-Bulle
    Pages 33-47

  6. Investigation of Optical Properties of Otoliths with Optical Trapping

    • Itia Amandine Favre-Bulle
    Pages 49-62

  7. Optical Manipulation of Otoliths In-Vivo

    • Itia Amandine Favre-Bulle
    Pages 63-84

  8. Conclusion

    • Itia Amandine Favre-Bulle
    Pages 85-86
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Keywords

About this book

The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

Authors and Affiliations

  • School of Mathematics and Physics, The University of Queensland, Brisbane, Australia

    Itia Amandine Favre‐Bulle

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