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  • © 2018

Advances in Plant Ecophysiology Techniques

  • Offers a unique, practical view on the most interesting parameters to study plant response to biotic/abiotic factors
  • Describes step-by-step laboratory-tested procedures for measuring ecophysiological parameters
  • Provides morphological, physiological, biochemical and molecular background information necessary for the methods used in the practical study of plant status

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Table of contents (28 chapters)

  1. Front Matter

    Pages i-xi
  2. In Vitro and In Vivo Bioassays

    • Mercedes Verdeguer
    Pages 1-13
  3. Root Morphology

    • Antonio Lupini, Fabrizio Araniti, Antonio Mauceri, Maria Princi, Antonino Di Iorio, Agostino Sorgonà et al.
    Pages 15-28
  4. Plant Photosynthetic Pigments: Methods and Tricks for Correct Quantification and Identification

    • Beatriz Fernández-Marín, José Ignacio García-Plazaola, Antonio Hernández, Raquel Esteban
    Pages 29-50
  5. Measuring Photosynthesis and Respiration with Infrared Gas Analysers

    • Cyril Douthe, Jorge Gago, Miquel Ribas-Carbó, Rubén Núñez, Nuria Pedrol, Jaume Flexas
    Pages 51-75
  6. Determining Plant Water Relations

    • Gorka Erice, María Luisa Pérez-Bueno, Mónica Pineda, Matilde Barón, Ricardo Aroca, Mónica Calvo-Polanco
    Pages 109-134
  7. Reactive Oxygen Species and Antioxidant Enzymatic Systems in Plants: Role and Methods

    • Teresa Papalia, Maria Rosaria Panuccio, Maria Sidari, Adele Muscolo
    Pages 177-193
  8. Flow Cytometric Measurement of Different Physiological Parameters

    • Fabrizio Araniti, Teodoro Coba de la Peña, Adela M. Sánchez-Moreiras
    Pages 195-213
  9. Flow Cytometry: Cell Cycle

    • Teodoro Coba de la Peña, Adela M. Sánchez-Moreiras
    Pages 215-229
  10. Mitotic Index

    • Elisa Graña
    Pages 231-240
  11. Fluorescent Probes and Live Imaging of Plant Cells

    • Elfrieda Fodor, Ferhan Ayaydin
    Pages 241-251
  12. Confocal and Transmission Electron Microscopy for Plant Studies

    • Adela M. Sánchez-Moreiras, Marianna Pacenza, Fabrizio Araniti, Leonardo Bruno
    Pages 253-271
  13. Visualization of Plant Microtubules

    • Elisa Graña
    Pages 283-294
  14. Multiprobe In-Situ Hybridization to Whole Mount Arabidopsis Seedlings

    • Leonardo Bruno, Fabrizio Araniti, Olimpia Gagliardi
    Pages 295-308
  15. Proteomics Analysis of Plant Tissues Based on Two-Dimensional Gel Electrophoresis

    • Jesus V. Jorrín-Novo, Luis Valledor-González, Mari A. Castillejo-Sánchez, Rosa Sánchez-Lucas, Isabel M. Gómez-Gálvez, Cristina López-Hidalgo et al.
    Pages 309-322

About this book

This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.

Editors and Affiliations

  • Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Adela M. Sánchez-Moreiras, Manuel J. Reigosa

About the editors









Bibliographic Information

  • Book Title: Advances in Plant Ecophysiology Techniques

  • Editors: Adela M. Sánchez-Moreiras, Manuel J. Reigosa

  • DOI: https://doi.org/10.1007/978-3-319-93233-0

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer International Publishing AG, part of Springer Nature 2018

  • Hardcover ISBN: 978-3-319-93232-3Published: 06 September 2018

  • Softcover ISBN: 978-3-030-06617-8Published: 29 December 2018

  • eBook ISBN: 978-3-319-93233-0Published: 17 August 2018

  • Edition Number: 1

  • Number of Pages: XI, 497

  • Number of Illustrations: 40 b/w illustrations, 70 illustrations in colour

  • Topics: Plant Ecology, Plant Physiology, Plant Biochemistry, Cell Physiology, Agriculture

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access