DNA Systems Under Internal and External Forcing

1. Front Matter

   Pages i-xv

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2. Introduction

   • Megan Clare Engel

   Pages 1-17

3. Simulation Methods

   • Megan Clare Engel

   Pages 19-24

4. Non-equilibrium Bio-Molecular Unfolding Under Tension

   • Megan Clare Engel

   Pages 25-67

5. Force-Induced Unravelling of DNA Origami

   • Megan Clare Engel

   Pages 69-93

6. Measuring Internal Forces in Single-Stranded DNA

   • Megan Clare Engel

   Pages 95-125

7. Conclusions

   • Megan Clare Engel

   Pages 127-130

8. Back Matter

   Pages 131-144

   PDF

The interactions of DNA with force are central to manifold fields of inquiry, including the de novo design of DNA nanostructures, the use of DNA to probe the principles of biological self-assembly, and the operation of cellular nanomachines. This work presents a survey of three distinct ways coarse-grained simulations can help characterize these interactions. A non-equilibrium energy landscape reconstruction technique is validated for use with the oxDNA model and a practical framework to guide future applications is established. A novel method for calculating entropic forces in DNA molecules is outlined and contrasted with existing, flawed approaches. Finally, a joint experimental-simulation study of large DNA origami nanostructures under force sheds light on design principles and, through vivid illustrations, their unfolding process. This text provides an accessible and exciting launching point for any student interested in the computational study of DNA mechanics and force interactions.

• Biological Self-Assembly
• DNA Nanotechnology
• DNA Coarse-Grained Modelling
• Non-Equilibrium Statistical Mechanics
• Jarzynski Equality
• DNA Origami
• Simulated Single Molecule Force Spectroscopy
• Folding Energy Landscapes
• OxDNA MODEL
• Entropic Polymer Forces
• Computing DNA self-assembly

• John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, USA

  Megan Clare Engel

Megan holds a DPhil in Theoretical Physics from the University of Oxford, which she completed on a Rhodes Scholarship, in addition to a BSc in Astrophysics with first class honours and a Masters in Physics, both from the University of Alberta in Canada. Her multidiscplinary interests have yielded diverse publications, including physics education research papers and book reviews in Science magazine, and she has worked in both experimental and theoretical capacities. Megan is currently a Killam postdoctoral fellow at the University of Alberta, where she continues to use coarse-grained modelling to explore how biological systems exploit physical laws.

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