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Peptide Nucleic Acids

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Peter E Nielsen

    1. Dept. Cellular & Molecular Medicine, University of Copenhagen Faculty of Health Sciences, Copenhagen N, Denmark

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    You can also search for this editor in PubMed   Google Scholar

  2. Daniel H. Appella

    1. NIH NIDDK, Bethesda, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Fully updated version of the popular first edition
  • Includes detailed, step-by-step protocols
  • Features notes on troubleshooting and avoiding known pitfalls

Part of the book series: Methods in Molecular Biology (MIMB, volume 1050)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. MiniPEG-γPNA

    • Arunava Manna, Srinivas Rapireddy, Raman Bahal, Danith H. Ly
    Pages 1-12

  3. Cyclopentane Peptide Nucleic Acids

    • Ethan A. Englund, Ning Zhang, Daniel H. Appella
    Pages 13-18

  4. Chiral PNAs with Constrained Open-Chain Backbones

    • Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Rosangela Marchelli
    Pages 19-35

  5. Synthesis, Characterization, and Evaluation of Radiometal-Containing Peptide Nucleic Acids

    • Holger Stephan, Christian Foerster, Gilles Gasser
    Pages 37-54

  6. Preparation of Metal-Containing Peptide Nucleic Acid Bioconjugates on the Solid Phase

    • Gilles Gasser
    Pages 55-72

  7. Formation and Characterization of PNA-Containing Heteroquadruplexes

    • Bruce A. Armitage
    Pages 73-82

  8. Sequence Selective Recognition of Double-Stranded RNA Using Triple Helix-Forming Peptide Nucleic Acids

    • Thomas Zengeya, Pankaj Gupta, Eriks Rozners
    Pages 83-94

  9. Assembly of PNA-Tagged Small Molecules, Peptides, and Carbohydrates onto DNA Templates: Programming the Combinatorial Pairing and Inter-ligand Distance

    • Nicolas Winssinger, Katarzyna Gorska, Mihai Ciobanu, Jean Pierre Daguer, Sofia Barluenga
    Pages 95-110

  10. Site-Selective Scission of Human Genome Using PNA-Based Artificial Restriction DNA Cutter

    • Kenichiro Ito, Makoto Komiyama
    Pages 111-120

  11. PNA Openers and Their Applications for Bacterial DNA Diagnostics

    • Irina V. Smolina, Maxim D. Frank-Kamenetskii
    Pages 121-130

  12. DNA-Templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids: A Versatile Tool for Single Base-Specific Detection of Nucleic Acids

    • Alexander Roloff, Simon Ficht, Christian Dose, Oliver Seitz
    Pages 131-141

  13. Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods

    • Stefano Sforza, Tullia Tedeschi, Mariangela Bencivenni, Alessandro Tonelli, Roberto Corradini, Rosangela Marchelli
    Pages 143-157

  14. Analysis of PNA Hybridization by Surface Plasmon Resonance

    • Bruce A. Armitage
    Pages 159-165

  15. PNA Fluorescent In Situ Hybridization (FISH) for Rapid Microbiology and Cytogenetic Analysis

    • Henrik Stender, Brett Williams, James Coull
    Pages 167-178

  16. Rapid miRNA Imaging in Cells Using Fluorogenic Templated Staudinger Reaction Between PNA-Based Probes

    • Katarzyna Gorska, Nicolas Winssinger
    Pages 179-192

  17. Cellular Delivery of Peptide Nucleic Acids (PNAs)

    • Takehiko Shiraishi, Peter E. Nielsen
    Pages 193-205

  18. Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification

    • Erica B. Schleifman, Peter M. Glazer
    Pages 207-222

  19. Antisense Effects of PNAs in Bacteria

    • Shan Goh, Jem Stach, Liam Good
    Pages 223-236

  20. Back Matter

    Pages 237-239
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Keywords

About this book

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.

Editors and Affiliations

  • Dept. Cellular & Molecular Medicine, University of Copenhagen Faculty of Health Sciences, Copenhagen N, Denmark

    Peter E Nielsen

  • NIH NIDDK, Bethesda, USA

    Daniel H. Appella

Bibliographic Information

  • Book Title: Peptide Nucleic Acids

  • Book Subtitle: Methods and Protocols

  • Editors: Peter E Nielsen, Daniel H. Appella

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-553-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-552-1Published: 04 December 2013

  • Softcover ISBN: 978-1-4939-5952-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-553-8Published: 02 December 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 239

  • Number of Illustrations: 39 b/w illustrations, 21 illustrations in colour

  • Topics: Biochemistry, general, Nucleic Acid Chemistry

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