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Mucins

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Michael A. McGuckin

    1. Mucosal Diseases Program, Mater Medical Research Institute, South Brisbane, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. David J. Thornton

    1. Fac. Life Sciences, Wellcome Trust Centre for, University of Manchester, Manchester, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Highlights the technical challenges while describing procedures
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 842)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins

    • Karine Rousseau, Dallas M. Swallow
    Pages 1-26

  3. Gel-Forming and Cell-Associated Mucins: Preparation for Structural and Functional Studies

    • Julia R. Davies, Claes Wickström, David J. Thornton
    Pages 27-47

  4. Detecting, Visualising, and Quantifying Mucins

    • Ceri A. Harrop, David J. Thornton, Michael A. McGuckin
    Pages 49-66

  5. Mass Spectrometric Analysis of Mucin Core Proteins

    • Mehmet Kesimer, John K. Sheehan
    Pages 67-79

  6. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

    • Thomas A. Gerken
    Pages 81-108

  7. Analysis of Assembly of Secreted Mucins

    • Malin E. V. Johansson, Gunnar C. Hansson
    Pages 109-121

  8. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling, and Release Through Exosomes

    • Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey
    Pages 123-140

  9. Glycomic Work-Flow for Analysis of Mucin O-Linked Oligosaccharides

    • Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunsheng Jin, Niclas G. Karlsson
    Pages 141-163

  10. O-Glycomics: Profi ling and Structural Analysis of Mucin-type O-linked Glycans

    • Isabelle Breloy
    Pages 165-177

  11. O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry

    • Franz-Georg Hanisch
    Pages 179-189

  12. Analysing Mucin Degradation

    • Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline M. Rudd, Elizabeth Matthews, Anthony P. Corfield
    Pages 191-215

  13. Assessment of Mucus Thickness and Production In Situ

    • Lena Holm, Mia Phillipson
    Pages 217-227

  14. Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH

    • Malin E. V. Johansson, Gunnar C. Hansson
    Pages 229-235

  15. Ex Vivo Measurements of Mucus Secretion by Colon Explants

    • Jenny K. Gustafsson, Henrik Sjövall, Gunnar C. Hansson
    Pages 237-243

  16. Establishment of Respiratory Air–Liquid Interface Cultures and Their Use in Studying Mucin Production, Secretion, and Function

    • David B. Hill, Brian Button
    Pages 245-258

  17. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

    • Lubna H. Abdullah, Cédric Wolber, Mehmet Kesimer, John K. Sheehan, C. William Davis
    Pages 259-277

  18. Assessment of Intracellular Mucin Content In Vivo

    • Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
    Pages 279-295

  19. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

    • Yong H. Sheng, Sumaira Z. Hasnain, Chin Wen Png, Michael A. McGuckin, Sara K. Lindén
    Pages 297-312

  20. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro

    • Pablo Argüeso, Ilene K. Gipson
    Pages 313-325
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Keywords

About this book

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.  In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques  for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.

Editors and Affiliations

  • Mucosal Diseases Program, Mater Medical Research Institute, South Brisbane, Australia

    Michael A. McGuckin

  • Fac. Life Sciences, Wellcome Trust Centre for, University of Manchester, Manchester, United Kingdom

    David J. Thornton

Bibliographic Information

  • Book Title: Mucins

  • Book Subtitle: Methods and Protocols

  • Editors: Michael A. McGuckin, David J. Thornton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-513-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-512-1Published: 19 January 2012

  • Softcover ISBN: 978-1-4939-5886-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-513-8Published: 21 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 329

  • Topics: Biochemistry, general, Proteomics

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