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Methods in Molecular Biology

PRINS and In Situ PCR Protocols

Editors: Pellestor, Franck (Ed.)

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  • ISBN 978-1-59745-068-3
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  • ISBN 978-1-61737-636-8
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About this book

Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.

Reviews

From Reviews of the First Edition...

"...essential for any laboratory contemplating exploiting this powerful technique." -Microbiology Today

"...[a] splendid little book which covers the field quite well,,," -FEBS Letters

"...accessible and very practical...recommend[ed]...to any laboratory with an operational PCR machine...a valuable addition to the methods literature." -Quarterly Review of Biology

"...well written...contains sufficient detail for a novice to successfully complete the experiment...recommend[ed]...for libraries as well as individual researchers interested in applying PRINS technology." -Doody's Health Sciences Book Review Journal

Table of contents (21 chapters)

  • New Rapid Multicolor PRINS Protocol

    Yan, Ju (et al.)

    Pages 3-13

  • PRINS Combined With Peptide Nucleic Acid Labeling

    Pellestor, Franck (et al.)

    Pages 15-22

  • PRINS Combined With Indirect Immunofluorescence

    Coullin, Philippe

    Pages 23-32

  • PRINS for the Detection of Unique Sequences

    Wachtel, Stephen S. (et al.)

    Pages 33-40

  • Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)

    Koch, Jørn

    Pages 41-48

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-59745-068-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for Mexico
  • ISBN 978-1-58829-549-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for Mexico
  • ISBN 978-1-61737-636-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
PRINS and In Situ PCR Protocols
Editors
  • Franck Pellestor
Series Title
Methods in Molecular Biology
Series Volume
334
Copyright
2006
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-068-3
DOI
10.1385/1597450685
Hardcover ISBN
978-1-58829-549-1
Softcover ISBN
978-1-61737-636-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 244
Topics