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Vaccinia Virus

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jason Mercer

    1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2023)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections with Poxviruses

    • Stuart N. Isaacs
    Pages 1-27

  3. Bioinformatics for Analysis of Poxvirus Genomes

    • Shin-Lin Tu, Chris Upton
    Pages 29-62

  4. Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis

    • Rachel L. Roper
    Pages 63-71

  5. Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins

    • N. Bishara Marzook, Timothy P. Newsome
    Pages 73-92

  6. Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines

    • Amber B. Rico, Annabel T. Olson, Matthew S. Wiebe
    Pages 93-108

  7. Vaccinia Virus Genome Editing Using CRISPR

    • Carmela Di Gioia, Ming Yuan, Yaohe Wang
    Pages 109-117

  8. RNAi-Mediated Depletion of Poxvirus Proteins

    • Caroline Martin, Samuel Kilcher
    Pages 119-130

  9. Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation

    • Nouhou Ibrahim, Paula Traktman
    Pages 131-141

  10. Preliminary Screening and In Vitro Confirmation of Orthopoxvirus Antivirals

    • Douglas W. Grosenbach, Dennis E. Hruby
    Pages 143-155

  11. Vaccinia Virus Transcriptome Analysis by RNA Sequencing

    • Shuai Cao, Yongquan Lin, Zhilong Yang
    Pages 157-170

  12. Ribosome Profiling of Vaccinia Virus-Infected Cells

    • Yongquan Lin, Wentao Qiao, Zhilong Yang
    Pages 171-188

  13. Quantitative PCR-Based Assessment of Vaccinia Virus RNA and DNA in Infected Cells

    • Moona Huttunen, Jason Mercer
    Pages 189-208

  14. Click Chemistry-Based Labeling of Poxvirus Genomes

    • Harriet Mok, Artur Yakimovich
    Pages 209-220

  15. Visualizing Poxvirus Replication and Recombination Using Live-Cell Imaging

    • Quinten Kieser, Patrick Paszkowski, James Lin, David Evans, Ryan Noyce
    Pages 221-235

  16. High-Content Analyses of Vaccinia Plaque Formation

    • Artur Yakimovich, Jason Mercer
    Pages 237-253

  17. Super-resolution Microscopy of Vaccinia Virus Particles

    • Robert Gray, David Albrecht
    Pages 255-268

  18. Bioluminescence Imaging as a Tool for Poxvirus Biology

    • Beatriz Perdiguero, Carmen Elena Gómez, Mariano Esteban
    Pages 269-285

  19. Growth and Purification of Vaccinia Virus Stocks for MPM Imaging

    • Glennys V. Reynoso, John P. Shannon, Jeffrey L. Americo, James Gibbs, Heather D. Hickman
    Pages 287-299

  20. Intravital Imaging of Vaccinia Virus-Infected Mice

    • John P. Shannon, Olena Kamenyeva, Glennys V. Reynoso, Heather D. Hickman
    Pages 301-311
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Keywords

About this book

This detailed book provides practical information for the laboratory that can be applied to the study of vaccinia and other poxviruses while emphasizing long-standing field standards and focusing on emerging new technologies applied in the field of poxvirology. The methods and protocols have been designed with the bench scientist in mind, being presented in a fashion that makes them useful for both starting and veteran poxvirus researchers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Vaccinia Virus: Methods and Protocols serves as a valuable resource for scientists looking to bring new methods and procedures into their lab in order to make exciting discoveries that will continue to deepen our understanding of this fascinating virus family.

Editors and Affiliations

  • MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

Bibliographic Information

  • Book Title: Vaccinia Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Jason Mercer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9593-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9592-9Published: 29 June 2019

  • Softcover ISBN: 978-1-4939-9595-0Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9593-6Published: 25 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 315

  • Number of Illustrations: 11 b/w illustrations, 31 illustrations in colour

  • Topics: Virology

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