Chiral Separations

1. Front Matter

   Pages i-xiv

   PDF

2. Recognition Mechanisms of Chiral Selectors: An Overview

   • Gerhard K. E. Scriba

   Pages 1-33

3. Enantioseparation by Thin-Layer Chromatography

   • Rituraj Dubey, Ravi Bhushan

   Pages 35-44

4. Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase

   • Sheng-Ming Xie, Jun-Hui Zhang, Li-Ming Yuan

   Pages 45-55

5. Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS

   • Toshimasa Toyo’oka

   Pages 57-79

6. Chiral Mobile-Phase Additives in HPLC Enantioseparations

   • Lushan Yu, Shengjia Wang, Su Zeng

   Pages 81-91

7. Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High-Performance Liquid Chromatography: An Overview

   • Bezhan Chankvetadze

   Pages 93-126

8. HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions

   • Roberto Cirilli

   Pages 127-146

9. Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC

   • Jie Zhou, Jian Tang, Weihua Tang

   Pages 147-157

10. HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases

    • Xiaoxuan Li, Yong Wang

    Pages 159-169

11. Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector

    • Liang Zhao, Hui Li, Shuqing Dong, Yanping Shi

    Pages 171-181

12. Cyclofructans as Chiral Selectors: An Overview

    • Garrett Hellinghausen, Daniel W. Armstrong

    Pages 183-200

13. High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview

    • István Ilisz, Tímea Orosz, Antal Péter

    Pages 201-237

14. Application of Sub-2 Micron Particle Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography

    • Chiara Fanali, Salvatore Fanali

    Pages 239-250

15. Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview

    • István Ilisz, Attila Bajtai, Antal Péter, Wolfgang Lindner

    Pages 251-277

16. Enantioseparations by High-Performance Liquid Chromatography Based on Chiral Ligand Exchange

    • Federica Ianni, Lucia Pucciarini, Andrea Carotti, Roccaldo Sardella, Benedetto Natalini

    Pages 279-302

17. Applications of Chiral Supercritical Fluid Chromatography

    • Emmanuelle Lipka

    Pages 303-319

18. Chiral Separations by Countercurrent Chromatography

    • Sheng-Qiang Tong

    Pages 321-337

19. Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations

    • Gerhard K. E. Scriba, Pavel Jáč

    Pages 339-356

20. Application of Dual-Cyclodextrin Systems in Capillary Electrophoresis Enantioseparations

    • Anne-Catherine Servais, Marianne Fillet

    Pages 357-364

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry.

• Liquid Chromatography
• Electrophoretic techniques
• Enantioseparations
• Chiral compounds
• Enantiomers
• Analytical separations

“The book is aimed at investigators in any discipline working in the field of chirality. It will be of special interest to anyone who has a need to separate chiral molecules. … Detailed protocols are provided for the systems covered in the book that will make it easier for investigators wishing to replicate one of these procedures.” (Thomas J. Wenzel, Analytical and Bioanalytical Chemistry, Vol. 411, 2019)

• Department of Pharmaceutical Chemistry, University of Jena, Jena, Germany

  Gerhard K. E. Scriba

Policies and ethics