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Radiation Cytogenetics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Takamitsu A. Kato

    1. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Paul F. Wilson

    1. Department of Radiation Oncology, University of California Davis, Sacramento, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1984)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis

    • Takamitsu A. Kato
    Pages 1-6

  3. Animal Lymphocyte Metaphase Chromosome Preparation

    • Kazuki Heishima, Kendon Kuo, Masashi Kimura, Takashi Mori
    Pages 7-22

  4. Micronuclei Formation Analysis After Ionizing Radiation

    • Cathy Su, Alexis H. Haskins, Takamitsu A. Kato
    Pages 23-29

  5. G1 Premature Chromosome Condensation (PCC) Assay

    • Ryuichi Okayasu, Cuihua Liu
    Pages 31-38

  6. G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity

    • Jeremy S. Haskins, Takamitsu A. Kato
    Pages 39-45

  7. G2 Premature Chromosome Condensation/Chromosome Aberration Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology

    • Eisuke Gotoh
    Pages 47-60

  8. Sister Chromatid Exchange as a Genotoxic Stress Marker

    • Shigeaki Sunada, Jeremy S. Haskins, Takamitsu A. Kato
    Pages 61-68

  9. DNA Damage Focus Formation Assay

    • Yoshihiro Fujii
    Pages 69-73

  10. Nuclear Foci Assays in Live Cells

    • Eiichiro Mori, Aroumougame Asaithamby
    Pages 75-85

  11. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes

    • Chisato Omata, Takamitsu A. Kato
    Pages 87-93

  12. PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations

    • Ian M. Cartwright, Jeremy S. Haskins, Takamitsu A. Kato
    Pages 95-100

  13. Modified PNA Telomere and Centromere FISH Protocols

    • Ian M. Cartwright
    Pages 101-105

  14. Directional Genomic Hybridization (dGH) for Detection of Intrachromosomal Rearrangements

    • Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey et al.
    Pages 107-116

  15. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH

    • Jeremy S. Haskins, Takamitsu A. Kato
    Pages 117-122

  16. Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting

    • Bradford D. Loucas
    Pages 123-135

  17. Back Matter

    Pages 137-138
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Keywords

About this book

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices workingon their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Editors and Affiliations

  • Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, USA

    Takamitsu A. Kato

  • Department of Radiation Oncology, University of California Davis, Sacramento, USA

    Paul F. Wilson

Bibliographic Information

  • Book Title: Radiation Cytogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Takamitsu A. Kato, Paul F. Wilson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9432-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9430-4Published: 04 July 2019

  • eBook ISBN: 978-1-4939-9432-8Published: 02 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 138

  • Number of Illustrations: 7 b/w illustrations, 11 illustrations in colour

  • Topics: Human Genetics, Cell Culture

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