Skip to main content
SpringerLink
Log in
Book cover

Acinetobacter baumannii

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Indranil Biswas

    1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Philip N. Rather

    1. Department of Microbiology and Immunology, Emory University, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1946)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp.

    • Binesh Lal, Saranya Vijayakumar, Shalini Anandan, Balaji Veeraraghavan
    Pages 1-15

  3. Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance

    • Saranya Vijayakumar, Balaji Veeraraghavan, Agila K. Pragasam, Yamuna Devi Bakthavachalam
    Pages 17-22

  4. Antimicrobial Susceptibility Testing Methods for Acinetobacter spp.

    • Balaji Veeraraghavan, Saranya Vijayakumar, Agila K. Pragasam, Yamuna Devi Bakthavachalam, John A. J. Prakash
    Pages 23-37

  5. Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii

    • Edgar X. Sherman, Jessie E. Wozniak, David S. Weiss
    Pages 39-50

  6. Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper-Supplemented Liquid Media and on Copper-Containing Surfaces

    • Caitlin L. Williams, D. Scott Merrell
    Pages 51-60

  7. A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy

    • Nithin Sam Ravi, Raziya Fathima Aslam, Balaji Veeraraghavan
    Pages 61-67

  8. Transformation of Acinetobacter baumannii: Electroporation

    • Mitchell G. Thompson, Süleyman Yildirim
    Pages 69-74

  9. Methods for Natural Transformation in Acinetobacter baumannii

    • Gottfried Wilharm, Evelyn Skiebe
    Pages 75-85

  10. Vesicle-Mediated Gene Transfer in Acinetobacter baumannii

    • Shweta Fulsundar, Sara Domingues, Kaare Magne Nielsen
    Pages 87-94

  11. Targeted Gene Replacement in Acinetobacter baumannii

    • Indranil Biswas, Joshua Mettlach
    Pages 95-106

  12. RecET-Mediated Recombineering in Acinetobacter baumannii

    • Ashley T. Tucker, Matthew J. Powers, M. Stephen Trent, Bryan W. Davies
    Pages 107-113

  13. Methods for Tn-Seq Analysis in Acinetobacter baumannii

    • Larry A. Gallagher
    Pages 115-134

  14. Tn7-Based Single-Copy Insertion Vectors for Acinetobacter baumannii

    • Kaleigh Ducas-Mowchun, P. Malaka De Silva, Rakesh Patidar, Herbert P. Schweizer, Ayush Kumar
    Pages 135-150

  15. Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii

    • Sarah E. Anderson, Philip N. Rather
    Pages 151-157

  16. A Simple Static Biofilm Assay for Acinetobacter baumannii

    • Indranil Biswas, Joshua Mettlach
    Pages 159-165

  17. BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth

    • Bertrand Naudin, Anja Heins, Stéphane Pinhal, Emmanuelle Dé, Marion Nicol
    Pages 167-176

  18. In Vitro Motility Assays for Acinetobacter Species

    • Indranil Biswas, Alexandra Machen, Joshua Mettlach
    Pages 177-187

  19. Desiccation Tolerance Assays for Acinetobacter baumannii

    • Xun Wang, M. Stephen Trent, Bryan W. Davies
    Pages 189-194

  20. Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure

    • Caitlin L. Williams, Heather M. Neu, Sarah L. J. Michel, D. Scott Merrell
    Pages 195-205
Back to top

Keywords

About this book

This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study.

Editors and Affiliations

  • Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, USA

    Indranil Biswas

  • Department of Microbiology and Immunology, Emory University, Atlanta, USA

    Philip N. Rather

Bibliographic Information

  • Book Title: Acinetobacter baumannii

  • Book Subtitle: Methods and Protocols

  • Editors: Indranil Biswas, Philip N. Rather

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9118-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9117-4Published: 24 February 2019

  • eBook ISBN: 978-1-4939-9118-1Published: 23 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 338

  • Number of Illustrations: 34 b/w illustrations, 53 illustrations in colour

  • Topics: Infectious Diseases, Bacteriology

Publish with us

Policies and ethics

Back to top

Search

Navigation