Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1922)
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Table of contents (41 protocols)
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Protocols for Establishment of Dental Cells Lines and Animal Models
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Protocols for Dental Stem Cells and Tissue Regeneration
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Protocols for Studying Gene and Protein Expression
Keywords
About this book
This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.
Editors and Affiliations
Bibliographic Information
Book Title: Odontogenesis
Book Subtitle: Methods and Protocols
Editors: Petros Papagerakis
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9012-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9011-5Published: 06 March 2019
eBook ISBN: 978-1-4939-9012-2Published: 05 March 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 565
Number of Illustrations: 17 b/w illustrations, 135 illustrations in colour
Topics: Molecular Medicine, Dentistry