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Experimental Models of Cardiovascular Diseases

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Kiyotake Ishikawa

    1. Icahn School of Medicine at Mount Sinai, New York, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key words and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1816)

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About this book

This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, leading to novel discoveries in cardiac biology,  and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Experimental Models of Cardiovascular Disease: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Keywords

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Experimental Models of Cardiovascular Diseases: Overview

      • Jae Gyun Oh, Kiyotake Ishikawa
      Pages 3-14

  3. In Silico Models

    1. Front Matter

      Pages 15-15
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    2. An Introduction to Computational Modeling of Cardiac Electrophysiology and Arrhythmogenicity

      • Joshua Mayourian, Eric A. Sobie, Kevin D. Costa
      Pages 17-35

  4. In Vitro Models

    1. Front Matter

      Pages 37-37
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    2. Isolation of Atrial and Ventricular Cardiomyocytes for In Vitro Studies

      • Jelena Plačkić, Jens Kockskämper
      Pages 39-54

    3. Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells

      • Adam T. Lynch, Silvia Mazzotta, Stefan Hoppler
      Pages 55-66

    4. Cardiomyocyte Differentiation from Human Embryonic Stem Cells

      • Silvia Mazzotta, Adam T. Lynch, Stefan Hoppler
      Pages 67-78

    5. Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes Using Embryoid Bodies

      • Takeshi Hatani, Kenji Miki, Yoshinori Yoshida
      Pages 79-92

    6. Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro

      • Przemek A. Gorski, Changwon Kho, Jae Gyun Oh
      Pages 93-104

  5. Ex Vivo Models

    1. Front Matter

      Pages 105-105
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    2. Langendorff Perfusion Method as an Ex Vivo Model to Evaluate Heart Function in Rats

      • Makino Watanabe, Takao Okada
      Pages 107-116

    3. Methods for the Preparation of an Excised, Cross-Circulated Rat Heart

      • Koji Obata, Miyako Takaki
      Pages 117-132

    4. Optical Action Potential Mapping in Acute Models of Ischemia–Reperfusion Injury: Probing the Arrhythmogenic Role of the Mitochondrial Translocator Protein

      • Zeki Ilkan, Benjamin Strauss, Chiara Campana, Fadi G. Akar
      Pages 133-143

    5. Cardiac Tissue Engineering Models of Inherited and Acquired Cardiomyopathies

      • Irene C. Turnbull, Joshua Mayourian, Jack F. Murphy, Francesca Stillitano, Delaine K. Ceholski, Kevin D. Costa
      Pages 145-159

    6. Badimon Perfusion Chamber: An Ex Vivo Model of Thrombosis

      • M. Urooj Zafar, Carlos G. Santos-Gallego, Lina Badimon, Juan J. Badimon
      Pages 161-171

  6. Small Animal Models

    1. Front Matter

      Pages 173-173
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    2. Ischemic Model of Heart Failure in Rats and Mice

      • Jiqiu Chen, Delaine K. Ceholski, Irene C. Turnbull, Lifan Liang, Roger J. Hajjar
      Pages 175-182

    3. Conventional Method of Transverse Aortic Constriction in Mice

      • Jimeen Yoo, Vadim Chepurko, Roger J. Hajjar, Dongtak Jeong
      Pages 183-193
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Editors and Affiliations

  • Icahn School of Medicine at Mount Sinai, New York, USA

    Kiyotake Ishikawa

Bibliographic Information

  • Book Title: Experimental Models of Cardiovascular Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Kiyotake Ishikawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8597-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8596-8Published: 09 July 2018

  • Softcover ISBN: 978-1-4939-9338-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8597-5Published: 09 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 404

  • Number of Illustrations: 25 b/w illustrations, 93 illustrations in colour

  • Topics: Molecular Medicine

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