Plant Chemical Genomics

1. Front Matter

   Pages i-xi

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2. Multi-Parametric Screening in Arabidopsis thaliana Seedlings

   • Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, Dominique Audenaert

   Pages 1-7

3. Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress

   • Susan Fozard, Brian G. Forde

   Pages 9-25

4. Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen

   • Roman Chuprov-Netochin, Elena Marusich, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Yan Ivanenkov et al.

   Pages 27-37

5. A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death Using Suspension Cell Culture of Arabidopsis thaliana

   • Yoshiteru Noutoshi, Ken Shirasu

   Pages 39-47

6. High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression

   • Vivek Halder, Erich Kombrink

   Pages 49-63

7. Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics

   • Clémence Bonnot, Laurent Nussaume, Thierry Desnos

   Pages 65-84

8. Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls Using BY-2 Cells

   • Emiko Okubo-Kurihara, Minami Matsui

   Pages 85-92

9. Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes

   • Mitsuhiro Kimura, Sachiko Isobe

   Pages 93-99

10. High-Throughput In Vitro Screening for Inhibitors of Cereal α-Glucosidase

    • Michael D. Rugen, Martin Rejzek, Henrik Naested, Birte Svensson, Robert A. Field

    Pages 101-115

11. Chemical Screening for Strigolactone Receptor Antagonists Using Arabidopsis thaliana

    • Duncan Holbrook-Smith, Peter McCourt

    Pages 117-126

12. Chemical Control of ABA Receptors to Enable Plant Protection Against Water Stress

    • Masanori Okamoto, Sean R. Cutler

    Pages 127-141

13. Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening

    • Yajin Ye, Yang Zhao

    Pages 143-148

14. Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside That Controls the Leaf Closure of Samanea saman

    • Yousuke Takaoka, Minoru Ueda

    Pages 149-158

15. Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule

    • Yoichi Takakusagi, Kaori Takakusagi, Fumio Sugawara, Kengo Sakaguchi

    Pages 159-172

16. Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants Using PlantiSMASH

    • Satria A. Kautsar, Hernando G. Suarez Duran, Marnix H. Medema

    Pages 173-188

17. Chemical Genomics Translatability from Unicellular to Multicellular Models

    • Cecilia Rodriguez-Furlán, Carlos Rubilar-Hernández, Lorena Norambuena

    Pages 189-201

18. Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii

    • Brittany Jack, Prachee Avasthi

    Pages 203-221

19. FnCpf1-Mediated Targeted Mutagenesis in Plants

    • Akira Endo, Seiichi Toki

    Pages 223-239

20. Erratum to: Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii

    • Brittany Jack, Prachee Avasthi

    Pages E1-E1

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.

• Arabidopsis
• reporter gene expression
• lignification
• Pi transporter
• P450 cytochrome

• Department of Molecular Biology, Princeton University, Princeton, USA

  Friedrich Fauser, Martin Jonikas

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