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MicroRNA Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Shao-Yao Ying

    1. Department of Integrative Anatomical Sciences, USC, Keck School of Medicine, Los Angeles, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1733)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The MicroRNA

    • Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin
    Pages 1-25

  3. Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro

    • Mainak Bose, Suvendra N. Bhattacharyya
    Pages 27-39

  4. Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection

    • Anna Majer, Stephanie A. Booth
    Pages 41-51

  5. Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid

    • Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma
    Pages 53-63

  6. Profiling of MicroRNAs in the Biofluids of Livestock Species

    • Jason Ioannidis, Judith Risse, F. Xavier Donadeu
    Pages 65-77

  7. Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders

    • Gabriel R. Fries, Joao Quevedo
    Pages 79-85

  8. Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation

    • Max Hübner, Pedro A. F. Galante, Simone Kreth, Ludwig Christian Hinske
    Pages 87-92

  9. How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells

    • Anna E. Zielak-Steciwko, John A. Browne
    Pages 93-105

  10. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

    • Shi-Lung Lin, Shao-Yao Ying
    Pages 107-126

  11. Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration

    • Sang-Ging Ong, Won Hee Lee, Yang Zhou, Joseph C. Wu
    Pages 127-136

  12. Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime

    • Nuno Bastos, Sónia A. Melo
    Pages 137-143

  13. Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets

    • Yoichi Robertus Fujii
    Pages 145-157

  14. In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis

    • Jian Xu, Xuelian Xiao, Daheng Yang
    Pages 159-171

  15. Isolation and Identification of Gene-Specific MicroRNAs

    • Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying
    Pages 173-180

  16. Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells

    • Masashi Fukuoka, Hirohiko Hohjoh
    Pages 181-192

  17. Screening miRNA for Functional Significance by 3D Cell Culture System

    • Bo Han
    Pages 193-201

  18. Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation

    • Lichan Tao, Yihua Bei, Yongqin Li, Junjie Xiao
    Pages 203-213

  19. Gene Manipulation with Micro RNAs at Single-Human Cancer Cell

    • Andres Stucky, Xuelian Chen, Jiang F. Zhong
    Pages 215-223

  20. Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis

    • Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro et al.
    Pages 225-237
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Keywords

About this book

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that’s cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource thatprovides researchers with a comprehensive study of miRNAs and its numerous components and uses.



Editors and Affiliations

  • Department of Integrative Anatomical Sciences, USC, Keck School of Medicine, Los Angeles, USA

    Shao-Yao Ying

Bibliographic Information

  • Book Title: MicroRNA Protocols

  • Editors: Shao-Yao Ying

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7601-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7600-3Published: 13 February 2018

  • Softcover ISBN: 978-1-4939-9250-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7601-0Published: 12 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 319

  • Number of Illustrations: 9 b/w illustrations, 41 illustrations in colour

  • Topics: Human Genetics

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