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Methods in Molecular Biology

Bacterial Chemosensing

Methods and Protocols

Editors: Manson, Michael D. (Ed.)

  • Includes cutting-edge methods and protocols for the study of bacterial chemosensing
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7577-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7576-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.

Table of contents (30 chapters)

  • The Diversity of Bacterial Chemosensing

    Manson, Michael D.

    Pages 3-6

  • Transmembrane Signal Transduction in Bacterial Chemosensing

    Manson, Michael D.

    Pages 7-19

  • Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay

    Korolik, Victoria (et al.)

    Pages 23-31

  • Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries

    Webb, Benjamin A. (et al.)

    Pages 33-45

  • A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients

    Sule, Nitesh (et al.)

    Pages 47-59

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7577-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7576-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Chemosensing
Book Subtitle
Methods and Protocols
Editors
  • Michael D. Manson
Series Title
Methods in Molecular Biology
Series Volume
1729
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7577-8
DOI
10.1007/978-1-4939-7577-8
Hardcover ISBN
978-1-4939-7576-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 389
Number of Illustrations
29 illustrations in colour
Topics