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Methods in Molecular Biology

mRNA Decay

Methods and Protocols

Editors: Lamandé, Shireen R. (Ed.)

  • Includes cutting-edge methods and protocols for the study of mRNA decay
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7540-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7539-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.

Table of contents (16 chapters)

  • 5′-Bromouridine IP Chase (BRIC)-Seq to Determine RNA Half-Lives

    Yamada, Toshimichi (et al.)

    Pages 1-13

  • Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)

    Abdul-Rahman, Farah (et al.)

    Pages 15-24

  • Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay

    Sun, Wei (et al.)

    Pages 25-34

  • Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH

    Trcek, Tatjana (et al.)

    Pages 35-54

  • PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins

    Garzia, Aitor (et al.)

    Pages 55-75

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7540-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7539-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
mRNA Decay
Book Subtitle
Methods and Protocols
Editors
  • Shireen R. Lamandé
Series Title
Methods in Molecular Biology
Series Volume
1720
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7540-2
DOI
10.1007/978-1-4939-7540-2
Hardcover ISBN
978-1-4939-7539-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 227
Number of Illustrations and Tables
10 b/w illustrations, 24 illustrations in colour
Topics