Skip to main content
  • Book
  • © 2018

Peptidomics

Methods and Strategies

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible products
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1719)

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvii
  2. Overview and Basic Techniques

    1. Front Matter

      Pages 1-1
    2. Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics

      • Elva Fridjonsdottir, Anna Nilsson, Henrik Wadensten, Per E. Andrén
      Pages 41-49
    3. Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry

      • Kazuki Sasaki, Takashi Tsuchiya, Tsukasa Osaki
      Pages 51-58
    4. Mass Spectrometric Identification of Endogenous Peptides

      • Mikel Azkargorta, Iraide Escobes, Ibon Iloro, Felix Elortza
      Pages 59-70
    5. Bioinformatics for Prohormone and Neuropeptide Discovery

      • Bruce R. Southey, Elena V. Romanova, Sandra L. Rodriguez-Zas, Jonathan V. Sweedler
      Pages 71-96
  3. Quantitative Methods in Peptidomics

    1. Front Matter

      Pages 119-119
    2. Quantitative Peptidomics with Isotopic and Isobaric Tags

      • Kurt Boonen, Wouter De Haes, Joris Van Houtven, Rik Verdonck, Geert Baggerman, Dirk Valkenborg et al.
      Pages 141-159
    3. Quantitative Peptidomics Using Reductive Methylation of Amines

      • Sayani Dasgupta, Leandro M. Castro, Alexandre K. Tashima, Lloyd Fricker
      Pages 161-174
    4. Metabolic Labeling to Quantify Drosophila Neuropeptides and Peptide Hormones

      • Thomas Otto Kunz, Jiangtian Chen, Megha, Christian Wegener
      Pages 175-185
  4. Diverse Applied Protocols and Outlook

    1. Front Matter

      Pages 197-197
    2. Neuropeptidomic Analysis of Zebrafish Brain

      • Kristien A. Van Camp, Geert Baggerman, Ronny Blust, Steven J. Husson
      Pages 241-246

About this book

This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls.

Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. 

Editors and Affiliations

  • Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Germany

    Michael Schrader

  • Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    Lloyd Fricker

Bibliographic Information

  • Book Title: Peptidomics

  • Book Subtitle: Methods and Strategies

  • Editors: Michael Schrader, Lloyd Fricker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7537-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7536-5Published: 24 February 2018

  • Softcover ISBN: 978-1-4939-8514-2Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7537-2Published: 23 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 423

  • Number of Illustrations: 62 b/w illustrations, 17 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access