Methods in Molecular Biology

Plant Membrane Proteomics

Methods and Protocols

Editors: Mock, Hans-Peter, Matros, Andrea, Witzel, Katja (Eds.)

  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7411-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7409-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.

Table of contents (10 chapters)

  • Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON

    Rainer Schwacke, Ulf-Ingo Flügge

    Pages

  • Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS

    Bin Li, et al.

    Pages

  • Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis

    Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty

    Pages

  • Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters

    Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood

    Pages

  • Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis

    Bronwyn J. Barkla

    Pages

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-7411-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for Mexico
  • ISBN 978-1-4939-7409-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Membrane Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Hans-Peter Mock
  • Andrea Matros
  • Katja Witzel
Series Title
Methods in Molecular Biology
Series Volume
1696
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7411-5
DOI
10.1007/978-1-4939-7411-5
Hardcover ISBN
978-1-4939-7409-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 280
Number of Illustrations and Tables
14 b/w illustrations, 33 illustrations in colour
Topics