- Includes cutting-edge methods and protocols for the study of plant germline development
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This detailed volume explores common and numerous specialized methods to study various aspects of plant germline development and targeted manipulation, including imaging and hybridization techniques to study cell-type specification, cell lineage, signaling and hormones, cell cycle, and the cytoskeleton. In addition, cell-type specific methods for targeted ablation or isolation are provided, protocols to apply “omics” technologies and to perform bioinformatics data analysis, as well as methods relevant for aspects of biotechnology or plant breeding. This includes protocols that are relevant for the targeted manipulation of pathways, for crop plant transformation, or for conditional induction of phenotypes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Germline Development: Methods and Protocols serves as a comprehensive guide not only to studying basic questions related to different aspects of plant reproductive development but also for state of the art methods, in addition to being a source of inspiration for new approaches and research questions in many laboratories.
- Table of contents (30 chapters)
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An Introduction to Male Germline Development
Pages 3-15
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Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants
Pages 17-34
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3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice
Pages 37-45
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Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana
Pages 47-54
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Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers
Pages 55-65
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Table of contents (30 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Germline Development
- Book Subtitle
- Methods and Protocols
- Editors
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- Anja Schmidt
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1669
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7286-9
- DOI
- 10.1007/978-1-4939-7286-9
- Hardcover ISBN
- 978-1-4939-7285-2
- Softcover ISBN
- 978-1-4939-8442-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 422
- Number of Illustrations
- 17 b/w illustrations, 86 illustrations in colour
- Topics
