Site-Specific Recombinases
Methods and Protocols
Editors: Eroshenko, Nikolai (Ed.)
Free Preview- Includes cutting-edge methods and protocolsProvides step-by-step detail for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
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This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
- Table of contents (20 chapters)
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Generating Genetically Modified Mice: A Decision Guide
Pages 1-19
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Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
Pages 21-35
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Building Cre Knockin Rat Lines Using CRISPR/Cas9
Pages 37-52
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Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
Pages 53-67
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Use of the DICE (Dual Integrase Cassette Exchange) System
Pages 69-85
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Site-Specific Recombinases
- Book Subtitle
- Methods and Protocols
- Editors
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- Nikolai Eroshenko
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1642
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7169-5
- DOI
- 10.1007/978-1-4939-7169-5
- Hardcover ISBN
- 978-1-4939-7167-1
- Softcover ISBN
- 978-1-4939-8405-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 342
- Number of Illustrations
- 25 b/w illustrations, 25 illustrations in colour
- Topics
