Methods in Molecular Biology

Optical Tweezers

Methods and Protocols

Editors: Gennerich, Arne (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-6421-5
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About this book

The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. 

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Introduction to Optical Tweezers

    Pages 3-24

    Koch, Matthias D. (et al.)

  • Exact Theory of Optical Tweezers and Its Application to Absolute Calibration

    Pages 25-39

    Dutra, Rafael S. (et al.)

  • Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes

    Pages 41-76

    Farré, Arnau (et al.)

  • A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization

    Pages 77-107

    Okoniewski, Stephen R. (et al.)

  • Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System

    Pages 109-136

    Bugiel, Michael (et al.)

Buy this book

eBook $109.00
price for Mexico
  • ISBN 978-1-4939-6421-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for Mexico
Softcover $139.00
price for Mexico
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Bibliographic Information

Bibliographic Information
Book Title
Optical Tweezers
Book Subtitle
Methods and Protocols
Editors
  • Arne Gennerich
Series Title
Methods in Molecular Biology
Series Volume
1486
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-6421-5
DOI
10.1007/978-1-4939-6421-5
Hardcover ISBN
978-1-4939-6419-2
Softcover ISBN
978-1-4939-8194-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 555
Number of Illustrations
21 b/w illustrations, 133 illustrations in colour
Topics