Overview
- Editors:
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Rafael Pulido
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BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain
- Includes cutting-edge methods and protocols involving the essentials of PTP function and regulation
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
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Table of contents (21 protocols)
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- Andrés Alonso, Caroline E. Nunes-Xavier, Yolanda Bayón, Rafael Pulido
Pages 1-23
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- Caroline E. Nunes-Xavier, Rafael Pulido
Pages 25-37
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- Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. Szedlacsek
Pages 39-66
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- Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, Rob Ruijtenbeek
Pages 67-78
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- Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens et al.
Pages 79-93
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- Laura Spinelli, Nicholas R. Leslie
Pages 95-105
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- Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz
Pages 107-119
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- Yunpeng Bai, Zhi-Hong Yu, Zhong-Yin Zhang
Pages 121-138
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- Colin Levy, James Adams, Lydia Tabernero
Pages 155-180
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- Wolfgang Peti, Rebecca Page
Pages 181-196
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- Christopher J. Caunt, Andrew M. Kidger, Stephen M. Keyse
Pages 197-215
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- Sina Koch, Irene Helbing, Sylvia-Annette Böhmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Söderberg et al.
Pages 217-242
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- Eynat Finkelshtein, Einat Levy-Apter, Ari Elson
Pages 283-300
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- Souad Rahmouni, Alexandre Hego, Céline Delierneux, Odile Wéra, Lucia Musumeci, Lutz Tautz et al.
Pages 301-330
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- Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnès Noël, Souad Rahmouni
Pages 331-349
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- Alexander James Hale, Jeroen den Hertog
Pages 351-372
About this book
This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methodsand Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
Editors and Affiliations
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BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain
Rafael Pulido