Protein Tyrosine Phosphatases
Methods and Protocols
Editors: Pulido, Rafael (Ed.)
Free Preview- Includes cutting-edge methods and protocols involving the essentials of PTP function and regulation
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
- Table of contents (21 chapters)
-
-
The Extended Family of Protein Tyrosine Phosphatases
Pages 1-23
-
Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome
Pages 25-37
-
Expression, Purification, and Kinetic Analysis of PTP Domains
Pages 39-66
-
Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples
Pages 67-78
-
Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B
Pages 79-93
-
Table of contents (21 chapters)
- Download Preface 1 PDF (72.7 KB)
- Download Sample pages 1 PDF (443.2 KB)
- Download Table of contents PDF (1.5 MB)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Protein Tyrosine Phosphatases
- Book Subtitle
- Methods and Protocols
- Editors
-
- Rafael Pulido
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1447
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3746-2
- DOI
- 10.1007/978-1-4939-3746-2
- Hardcover ISBN
- 978-1-4939-3744-8
- Softcover ISBN
- 978-1-4939-8127-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 402
- Number of Illustrations
- 28 b/w illustrations, 51 illustrations in colour
- Topics
