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Methods in Molecular Biology

Protein Tyrosine Phosphatases

Methods and Protocols

Editors: Pulido, Rafael (Ed.)

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  • Includes cutting-edge methods and protocols involving the essentials of PTP function and regulation
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-3746-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for Mexico
  • ISBN 978-1-4939-3744-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for Mexico
  • ISBN 978-1-4939-8127-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • The Extended Family of Protein Tyrosine Phosphatases

    Pages 1-23

    Alonso, Andrés (et al.)

  • Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome

    Pages 25-37

    Nunes-Xavier, Caroline E. (et al.)

  • Expression, Purification, and Kinetic Analysis of PTP Domains

    Pages 39-66

    Mentel, Mihaela (et al.)

  • Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples

    Pages 67-78

    Hovestad-Bijl, Liesbeth (et al.)

  • Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B

    Pages 79-93

    Luna, Sandra (et al.)

Buy this book

eBook $109.00
price for Mexico (gross)
  • ISBN 978-1-4939-3746-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for Mexico
  • ISBN 978-1-4939-3744-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for Mexico
  • ISBN 978-1-4939-8127-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Tyrosine Phosphatases
Book Subtitle
Methods and Protocols
Editors
  • Rafael Pulido
Series Title
Methods in Molecular Biology
Series Volume
1447
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3746-2
DOI
10.1007/978-1-4939-3746-2
Hardcover ISBN
978-1-4939-3744-8
Softcover ISBN
978-1-4939-8127-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 402
Number of Illustrations
28 b/w illustrations, 51 illustrations in colour
Topics