CYBER DEAL: 50% off all Springer eBooks | Get this offer!

Methods in Molecular Biology

Cyclin-Dependent Kinase (CDK) Inhibitors

Methods and Protocols

Editors: Orzaez, Mar, Sancho Medina, Mónica, Pérez-Payá, Enrique (Eds.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for Mexico
  • ISBN 978-1-4939-2926-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for Mexico
Softcover $119.00
price for Mexico
About this book

This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Immunoprecipitation of Cdk–Cyclin Complexes for Determination of Kinase Activity

    Pages 1-8

    Gallastegui, Edurne (et al.)

  • Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation

    Pages 9-12

    Gallastegui, Edurne (et al.)

  • Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9

    Pages 13-28

    Pinhero, Reena (et al.)

  • Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination

    Pages 29-45

    Grigoroudis, Asterios I. (et al.)

  • Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors

    Pages 47-58

    Tripathi, Sunil Kumar (et al.)

Buy this book

eBook $89.00
price for Mexico
  • ISBN 978-1-4939-2926-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for Mexico
Softcover $119.00
price for Mexico
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Cyclin-Dependent Kinase (CDK) Inhibitors
Book Subtitle
Methods and Protocols
Editors
  • Mar Orzaez
  • Mónica Sancho Medina
  • Enrique Pérez-Payá
Series Title
Methods in Molecular Biology
Series Volume
1336
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-2926-9
DOI
10.1007/978-1-4939-2926-9
Hardcover ISBN
978-1-4939-2925-2
Softcover ISBN
978-1-4939-4368-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 227
Number of Illustrations
20 b/w illustrations, 22 illustrations in colour
Topics