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Nuclear Reprogramming

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kejin Hu

    1. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2239)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Pig Cloning Using Somatic Cell Nuclear Transfer

    • Hongsheng Ouyang, Jianyong Han, Yongye Huang
    Pages 1-18

  3. Cloning of Monkeys by Somatic Cell Nuclear Transfer

    • Shiyu Sun, Zhaodi Liao, Zhen Liu, Qiang Sun
    Pages 19-31

  4. Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors

    • Emre Bektik, Ji-Dong Fu
    Pages 33-46

  5. Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media

    • Xiaowen Wang, Conrad P. Hodgkinson, Victor J. Dzau
    Pages 47-59

  6. Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-Free Cells

    • Minhyung Lee, Janghwan Kim, Rajesh Ambasudhan
    Pages 61-75

  7. Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts

    • Victoria A. Church, Kitra Cates, Lucia Capano, Shivani Aryal, Woo Kyung Kim, Andrew S. Yoo
    Pages 77-100

  8. Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium

    • Zhicheng Shao, Ricardo Raúl Cevallos, Kejin Hu
    Pages 101-116

  9. Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency

    • Wei Wen, Tao Cheng, Xiao-Bing Zhang
    Pages 117-133

  10. Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells

    • Fabia Febbraro, Muwan Chen, Mark Denham
    Pages 135-151

  11. Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling

    • Chun Liu, Mohamed Ameen, Sukaina Himmati, Dilip Thomas, Nazish Sayed
    Pages 153-162

  12. Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA

    • Robert R. Annand
    Pages 163-174

  13. Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators

    • Jere Weltner, Ras Trokovic
    Pages 175-198

  14. Reprogramming Porcine Fibroblast to EPSCs

    • Xuefei Gao, Degong Ruan, Pentao Liu
    Pages 199-211

  15. Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance

    • Jennifer L. Brehm, Molly M. Miles, Katie S. Remondini, Tenneille E. Ludwig
    Pages 213-234

  16. Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers

    • Ricardo Raúl Cevallos, Md Emon Hossain, Ruowen Zhang, Kejin Hu
    Pages 235-249

  17. G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures

    • Erik McIntire, Kimberly Leonhard, Seth Taapken, Anna Lisa Larson
    Pages 251-268

  18. Become Competent in Generating RNA-Seq Heat Maps in One Day for Novices Without Prior R Experience

    • Kejin Hu
    Pages 269-303

  19. Back Matter

    Pages 305-306
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Keywords

About this book

This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.

 

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, USA

    Kejin Hu

Bibliographic Information

  • Book Title: Nuclear Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Kejin Hu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1084-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1083-1Published: 24 November 2020

  • Softcover ISBN: 978-1-0716-1086-2Published: 23 November 2020

  • eBook ISBN: 978-1-0716-1084-8Published: 23 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 306

  • Number of Illustrations: 19 b/w illustrations, 54 illustrations in colour

  • Topics: Nucleic Acid Chemistry, Stem Cells

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