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Reactive Oxygen Species

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2202)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Assessment of ROS Production in the Mitochondria of Live Cells

    • Plamena R. Angelova, Albena T. Dinkova-Kostova, Andrey Y. Abramov
    Pages 33-42
  3. Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy

    • Pablo Hernansanz-Agustín, Carmen Choya-Foces, Antonio Martínez-Ruiz
    Pages 43-50
  4. A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in Ex Vivo Culture

    • María I. Calvo-Sánchez, Sandra Fernández-Martos, Jesús Espada
    Pages 51-61
  5. Characterization of Oxidative Lipidomics and Autophagy Induction in Chlamydomonas reinhardtii Under Abiotic Stress

    • Cristina Ortega-Villasante, Ángel Barón-Sola, Margarita Toledo-Basantes, Flor Martínez, Luis E. Hernández
    Pages 71-80
  6. Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa

    • Vanesa Robles, Marta F. Riesco, Juan M. Martínez-Vázquez, David G. Valcarce
    Pages 93-102
  7. EPR Spectroscopy as a Method for ROS Quantification in the Skin

    • Silke B. Lohan, Daniela Ivanov, Nadine Schüler, Bastian Berger, Stephanie Albrecht, Martina C. Meinke
    Pages 137-148
  8. Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors

    • Roger Bresolí-Obach, Joaquim Torra, Renzo P. Zanocco, Antonio L. Zanocco, Santi Nonell
    Pages 165-188
  9. The Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy

    • Sulbha K. Sharma, Michael R. Hamblin
    Pages 215-229
  10. Back Matter

    Pages 231-233

About this book

This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile, Madrid, Spain

    Jesús Espada

Bibliographic Information

  • Book Title: Reactive Oxygen Species

  • Book Subtitle: Methods and Protocols

  • Editors: Jesús Espada

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0896-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0895-1Published: 29 August 2020

  • Softcover ISBN: 978-1-0716-0898-2Published: 29 August 2021

  • eBook ISBN: 978-1-0716-0896-8Published: 28 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 233

  • Number of Illustrations: 21 b/w illustrations, 42 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access