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Methods in Molecular Biology
cover

Metabolic Flux Analysis in Eukaryotic Cells

Methods and Protocols

Editors: Nagrath, Deepak (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0159-4
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About this book

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Determination of Isotopologue and Tandem Mass Isotopologue Ratios Using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry - Methodology and Uncertainty of Measurement

    Pages 1-16

    Mairinger, Teresa (et al.)

  • Non-Targeted Mass Isotopolome Analysis Using Stable Isotope Patterns to Identify Metabolic Changes

    Pages 17-32

    Dudek, Christian-Alexander (et al.)

  • Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Metabolic Flux Analysis

    Pages 33-50

    Jaiswal, Damini (et al.)

  • Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cells

    Pages 51-71

    Lim, Esther W. (et al.)

  • Large-Scale Profiling of Cellular Metabolic Activities Using Deep 13C Labeling Medium

    Pages 73-92

    Grankvist, Nina (et al.)

Buy this book

eBook $169.00
price for Mexico (gross)
  • ISBN 978-1-0716-0159-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for Mexico
  • ISBN 978-1-0716-0158-7
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Metabolic Flux Analysis in Eukaryotic Cells
Book Subtitle
Methods and Protocols
Editors
  • Deepak Nagrath
Series Title
Methods in Molecular Biology
Series Volume
2088
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0159-4
DOI
10.1007/978-1-0716-0159-4
Hardcover ISBN
978-1-0716-0158-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 370
Number of Illustrations
23 b/w illustrations, 64 illustrations in colour
Topics

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