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Extracellular RNA

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Tushar Patel

    1. Department of Transplantation, Mayo Clinic, Jacksonville, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1740)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Extracellular RNAs: A New Awareness of Old Perspectives

    • Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler et al.
    Pages 1-15

  3. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles

    • Julia Small, Sabrina Roy, Roger Alexander, Leonora Balaj
    Pages 17-21

  4. Extracellular RNA Isolation from Cell Culture Supernatant

    • Sawen Bakr, Bridget Simonson, Kirsty M. Danielson, Saumya Das
    Pages 23-34

  5. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture

    • Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar Patel
    Pages 35-41

  6. Isolation of Extracellular RNA from Serum/Plasma

    • Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang et al.
    Pages 43-57

  7. Isolation of Extracellular RNA from Bile

    • Irene K. Yan, Valentin X. Berdah, Tushar Patel
    Pages 59-67

  8. Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes

    • Kang Li, David K. Wong, King Yeung Hong, Robert L. Raffai
    Pages 69-83

  9. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids

    • Pete Heinzelman
    Pages 85-107

  10. Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography

    • Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, Joshua N. Leonard
    Pages 109-124

  11. Detection and Analysis of Non-vesicular Extracellular RNA

    • Juan Pablo Tosar, Alfonso Cayota
    Pages 125-137

  12. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA

    • Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, Robert L. Raffai
    Pages 139-153

  13. Droplet Digital PCR for Quantitation of Extracellular RNA

    • Irene K. Yan, Rishabh Lohray, Tushar Patel
    Pages 155-162

  14. Preparation of Small RNA NGS Libraries from Biofluids

    • Alton Etheridge, Kai Wang, David Baxter, David Galas
    Pages 163-175

  15. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString

    • Neha Shukla, Irene K. Yan, Tushar Patel
    Pages 177-185

  16. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs

    • Akiko Matsuda, Tushar Patel
    Pages 187-197

  17. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs

    • Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, Anastasia Khvorova
    Pages 199-214

  18. Back Matter

    Pages 215-216
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Keywords

About this book

This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Editors and Affiliations

  • Department of Transplantation, Mayo Clinic, Jacksonville, USA

    Tushar Patel

Bibliographic Information

  • Book Title: Extracellular RNA

  • Book Subtitle: Methods and Protocols

  • Editors: Tushar Patel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7652-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7651-5Published: 01 February 2018

  • Softcover ISBN: 978-1-4939-8535-7Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7652-2Published: 30 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 216

  • Number of Illustrations: 13 b/w illustrations, 30 illustrations in colour

  • Topics: Human Genetics

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