Cryptosporidium

1. Front Matter

   Pages i-xi

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2. Cryptosporidium Diagnostic Assays: Microscopy

   • Guy Robinson, Rachel M. Chalmers

   Pages 1-10

3. Cryptosporidium Diagnostic Assays: Molecular Detection

   • Guy Robinson, Kristin Elwin, Rachel M. Chalmers

   Pages 11-22

4. Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

   • Amy M. Kahler, Vincent R. Hill

   Pages 23-41

5. Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation

   • Michael J. Arrowood

   Pages 43-59

6. Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

   • Jeffrey W. Priest, Delynn M. Moss

   Pages 61-85

7. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

   • Iwona Driskell, Roberta M. O’Connor

   Pages 87-102

8. Cryptosporidium Genotyping for Epidemiology Tracking

   • Dawn M. Roellig, Lihua Xiao

   Pages 103-116

9. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection

   • Anson V. Koehler, Jan Šlapeta

   Pages 117-127

10. Preparation of Cryptosporidium DNA for Whole Genome Sequencing

    • Guy Robinson, Rachel M. Chalmers

    Pages 129-138

11. Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org

    • Susanne Warrenfeltz, Jessica C. Kissinger, On Behalf of the EuPathDB Team

    Pages 139-192Open Access

12. A Novel Method to Silence Genes in Cryptosporidium

    • Alejandro Castellanos-Gonzalez

    Pages 193-203

13. Use of miRNAs to Study Host Cell–Parasite Interactions

    • Ji-Hao Xu, Nicholas W. Mathy, Xian-Ming Chen

    Pages 205-218

14. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

    • Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen

    Pages 219-228

15. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

    • Karine Sonzogni-Desautels, Jan R. Mead, Momar Ndao

    Pages 229-251

16. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics

    • Michael W. Riggs, Deborah A. Schaefer

    Pages 253-282

17. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

    • Jennifer L. Murphy, Michael J. Arrowood

    Pages 283-302

18. High-Content Screening for Cryptosporidium Drug Discovery

    • Melissa S. Love, Case W. McNamara

    Pages 303-317

19. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR

    • Haili Zhang, Guan Zhu

    Pages 319-334

20. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds

    • Nigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, Samuel Arnold

    Pages 335-350

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.


The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

• Protozoan
• Intestinal tract infection
• Anti-cryptosporidial drugs
• Genotyping
• Oocysts
• CRISPR/Cas9
• Host-parasite interactions
• infectious diseases

• Atlanta Veterans Affairs Medical Center, Decatur, USA

  Jan R. Mead

• Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA

  Michael J. Arrowood

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