Genotoxicity Assessment

1. Front Matter

   Pages i-xi

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2. Gene Mutation Assays

   1. Front Matter

      Pages 1-1

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   2. In Vitro Mouse Lymphoma Cell (L5178Y Tk^+/− −3.7.2.C) Forward Mutation Assay

      • Melissa R. Schisler, Martha M. Moore, B. Bhaskar Gollapudi

      Pages 3-28

   3. Erythrocyte-Based Pig-a Gene Mutation Assay

      • Jeffrey C. Bemis, Nikki E. Hall, Stephen D. Dertinger

      Pages 29-57

   4. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

      • Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, Robert H. Heflich

      Pages 59-75

3. Assays for Chromosomal Abnormalities

   1. Front Matter

      Pages 77-77

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   2. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

      • Pasquale Mosesso, Serena Cinelli

      Pages 79-104

   3. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry

      • Antonella Testa, Valentina Palma, Clarice Patrono

      Pages 105-119

   4. Chromosomal Aberration Test in Human Lymphocytes

      • Christian Johannes, Guenter Obe

      Pages 121-134

   5. In Vivo Micronucleus Assay in Mouse Bone Marrow

      • Abhishek Kumar Jain, Alok Kumar Pandey

      Pages 135-146

   6. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells

      • Claudia Bolognesi, Michael Fenech

      Pages 147-163

   7. Micronucleus Analysis by Flow Cytometry

      • Azeddine Elhajouji, Pascal Stadelmann

      Pages 165-193

   8. Hen’s Egg Test for Micronucleus Induction (HET-MN)

      • Kerstin Reisinger, Eva Dony, Thorsten Wolf, Katrin Maul

      Pages 195-208

   9. Comparative Genomic Hybridization (CGH) in Genotoxicology

      • Adi Baumgartner, Veronika Hartleb, Jim D. Taylor

      Pages 209-234

4. Tests for Primary DNA Damage

   1. Front Matter

      Pages 235-235

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   2. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

      • Mahima Bajpayee, Ashutosh Kumar, Alok Dhawan

      Pages 237-257

   3. The Comet Assay in Human Biomonitoring

      • Diana Anderson, Alok Dhawan, Julian Laubenthal

      Pages 259-274

   4. The Comet Assay in Marine Animals

      • Patrizia Guidi, Brett P. Lyons, Giada Frenzilli

      Pages 275-286

   5. Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

      • Julian Laubenthal, Michal R. Gdula, Alok Dhawan, Diana Anderson

      Pages 287-300

   6. Detection of DNA Double-Strand Breaks Using Pulsed-Field Gel Electrophoresis

      • Vyom Sharma, Vishwa Mohan

      Pages 301-311

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

• Genetic toxicology
• Mutation assays
• DNA damage
• Chromosomal abnormalities
• Toxicogenomics
• Computational predictive models
• ecotoxicology

• CSIR—Indian Institute of Toxicology Research, Lucknow, India

  Alok Dhawan

• New Delhi, India

  Mahima Bajpayee

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