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Immunoproteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2024)

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Softcover Book USD 139.99
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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction to the Immune System

    • Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, Felicity Stark
    Pages 1-24
  3. Immunoproteomics Methods and Techniques

    • Kelly M. Fulton, Isabel Baltat, Susan M. Twine
    Pages 25-58
  4. Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification

    • Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, Susan M. Twine
    Pages 59-78
  5. Enriching for Low-Abundance Serum Proteins Using ProteoMinerâ„¢ and Protein-Level HPLC

    • Sophie Moggridge, Kelly M. Fulton, Susan M. Twine
    Pages 103-117
  6. Identification of the Antigen Content of Electroimmunoprecipitates

    • N. Helena Beyer, Niels H. H. Heegaard
    Pages 119-135
  7. Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum

    • Christie E. Delaney, John F. Kelly, Wen Ding, Arsalan S. Haqqani
    Pages 153-166
  8. Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

    • Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall
    Pages 199-211
  9. Enhanced Mass Spectrometry Detection of MHC Peptides

    • Rui Chen, Jianjun Li
    Pages 245-257
  10. Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach

    • Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, Shashi Gujar
    Pages 301-307

About this book

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field. 


Editors and Affiliations

  • Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Canada

    Kelly M. Fulton, Susan M. Twine

Bibliographic Information

  • Book Title: Immunoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Kelly M. Fulton, Susan M. Twine

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9597-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9596-7Published: 31 July 2019

  • Softcover ISBN: 978-1-4939-9599-8Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9597-4Published: 30 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 380

  • Number of Illustrations: 18 b/w illustrations, 57 illustrations in colour

  • Topics: Immunology, Proteomics

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access