Overview
- Editors:
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Tariq M. Haqqi
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Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, USA
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VĂ©ronique Lefebvre
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Department of Surgery, The Children's Hospital of Philadelphia, Philadelphia, USA
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (15 protocols)
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Front Matter
Pages i-xiii
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- Rodrigo A. Somoza, Jean F. Welter
Pages 1-12
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- Ilona Uzieliene, Jaroslav Denkovskij, Eiva Bernotiene, Ursule Kalvaityte, Raminta Vaiciuleviciute, Yolande F. M. Ramos et al.
Pages 13-22
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- Ioannis Kanakis, Mohammad Alhashmi, Ke Liu, Craig Keenan, Lorenzo Ramos Mucci, Blandine Poulet et al.
Pages 23-38
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- Abdul Haseeb, VĂ©ronique Lefebvre
Pages 39-51
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- Zachary Tata, Christophe Merceron, Ernestina Schipani
Pages 53-84
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- Charles de Charleroy, Abdul Haseeb, VĂ©ronique Lefebvre
Pages 85-92
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- Helal Endisha, Mohit Kapoor
Pages 93-103
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- Bijal Kikani, Julian C. Lui
Pages 105-119
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- Hongjun Zheng, Audrey McAlinden
Pages 121-133
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- Mandy J. Peffers, Andy Cremers, Tim J. M. Welting
Pages 135-149
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- Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz et al.
Pages 151-166
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- Akira Yamakawa, Hironori Hojo, Shinsuke Ohba
Pages 167-178
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- Yolande F. M. Ramos, Ali Mobasheri
Pages 179-194
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- Yun Wang, Liang-Yu Chen, Ru Liu-Bryan
Pages 195-213
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- Mohammad Y. Ansari, Tariq M. Haqqi
Pages 215-224
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Back Matter
Pages 225-229
About this book
This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chondrocytes: Methods andProtocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.
Editors and Affiliations
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Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, USA
Tariq M. Haqqi
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Department of Surgery, The Children's Hospital of Philadelphia, Philadelphia, USA
VĂ©ronique Lefebvre