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DNA Cloning and Assembly

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Sunil Chandran

    1. Amyris, Inc., Emeryville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Kevin W. George

    1. Amyris, Inc., Emeryville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2205)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. In Silico Tools for DNA Design and Assembly

    1. Front Matter

      Pages 1-1
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    2. An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology

      • Ernst Oberortner, Robert Evans, Xianwei Meng, Sangeeta Nath, Hector Plahar, Lisa Simirenko et al.
      Pages 3-18

    3. Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5

      • Michael J. Fero, James K. Craft, Trang Vu, Nathan J. Hillson
      Pages 19-47

    4. The AssemblX Toolkit for Reliable and User-Friendly Multigene Assemblies

      • Fabian Machens, Lena Hochrein
      Pages 49-67

  3. High Throughput Workflows

    1. Front Matter

      Pages 69-69
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    2. High-Throughput PCR for DNA Part Generation

      • David Reif
      Pages 71-77

    3. High-Throughput DNA Assembly Using Yeast Homologous Recombination

      • Kristy Ip, Ron Yadin, Kevin W. George
      Pages 79-89

    4. Highly Multiplexed, Semiautomated Nextera Next-Generation Sequencing (NGS) Library Preparation

      • William Christie, Ron Yadin, Kristy Ip, Kevin W. George
      Pages 91-104

  4. DNA Assembly Standards

    1. Front Matter

      Pages 105-105
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    2. Generation of MoClo Standard Parts Using Golden Gate Cloning

      • Ramona Grützner, Sylvestre Marillonnet
      Pages 107-123

    3. Assembly of Multigene Constructs Using the Modular Cloning System MoClo

      • Sylvestre Marillonnet, Stefan Werner
      Pages 125-141

    4. Hierarchical Modular DNA Assembly Using MetClo

      • Da Lin, Christopher A. O’Callaghan
      Pages 143-159

    5. PaperClip DNA Assembly: Reduce, Reuse, Recycle

      • Maryia Trubitsyna, Annegret Honsbein, Uma Jayachandran, Alistair Elfick, Christopher E. French
      Pages 161-177

    6. Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants

      • Yao-Min Cai, Jose A. Carrasco Lopez, Nicola J. Patron
      Pages 179-199

    7. Mobius Assembly

      • Andreas I. Andreou, Naomi Nakayama
      Pages 201-218

    8. Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly

      • George M. Taylor, John T. Heap
      Pages 219-237

    9. BASIC: A Simple and Accurate Modular DNA Assembly Method

      • Marko Storch, Ari Dwijayanti, Haris Mallick, Matthew C. Haines, Geoff S. Baldwin
      Pages 239-253

    10. Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host

      • Marcos Valenzuela-Ortega, Christopher E. French
      Pages 255-273

  5. Combinatorial Methods for Pathway Optimization

    1. Front Matter

      Pages 275-275
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Keywords

About this book

This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Amyris, Inc., Emeryville, USA

    Sunil Chandran, Kevin W. George

Bibliographic Information

  • Book Title: DNA Cloning and Assembly

  • Book Subtitle: Methods and Protocols

  • Editors: Sunil Chandran, Kevin W. George

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0908-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0907-1Published: 19 August 2020

  • Softcover ISBN: 978-1-0716-0910-1Published: 19 August 2021

  • eBook ISBN: 978-1-0716-0908-8Published: 18 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 334

  • Number of Illustrations: 12 b/w illustrations, 69 illustrations in colour

  • Topics: Human Genetics

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