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The Nucleus

  • Book
  • © 2020

Overview

Editors:
  1. Ronald Hancock

    1. Biosystems Group, Biotechnology Center, Silesian University of Technology, Gliwice, Poland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2175)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Live-Cell Imaging and Analysis of Nuclear Body Mobility

    • Dmitry V. Sorokin, Eugene A. Arifulin, Yegor S. Vassetzky, Eugene V. Sheval
    Pages 1-9

  3. Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell

    • David Anchel, Reagan W. Ching
    Pages 11-21

  4. Visualizing Chromatin Modifications in Isolated Nuclei

    • Yuan Li, Zachary Klase, Luca Sardo
    Pages 23-31

  5. Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture

    • Alexey I. Chizhik, Anna M. Chizhik, Daja Ruhlandt, Janine Pfaff, Narain Karedla, Ingo Gregor et al.
    Pages 33-45

  6. Studying Proton Gradients Across the Nuclear Envelope

    • Raul Martínez-Zaguilán, Souad R. Sennoune
    Pages 47-63

  7. COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes

    • Michael Hausmann, Jin-Ho Lee, Aaron Sievers, Matthias Krufczik, Georg Hildenbrand
    Pages 65-77

  8. Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq

    • Peng Mao, John J. Wyrick
    Pages 79-94

  9. AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide

    • Anna R. Poetsch
    Pages 95-108

  10. Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID

    • Enes Ugur, Michael D. Bartoschek, Heinrich Leonhardt
    Pages 109-121

  11. Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions

    • David Umlauf, Michal Sobecki, Laure Crabbe
    Pages 123-138

  12. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

    • Šárka SchoÅ™ová, Jiří Fajkus, Petra Procházková Schrumpfová
    Pages 139-167

  13. Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells

    • Sara N. Mouton, Liesbeth M. Veenhoff, Arnold J. Boersma
    Pages 169-180

  14. Analysis of a Nuclear Intrinsically Disordered Proteome

    • Bozena Skupien-Rabian, Urszula Jankowska, Sylwia Kedracka-Krok
    Pages 181-196

  15. Timing of Cytosine Methylation on Newly Synthesized RNA by Electron Microscopy

    • Lorena Zannino, Stella Siciliani, Marco Biggiogera
    Pages 197-205

  16. The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants

    • Annette Piechulek, Lutz Berwanger, Peter Hemmerich, Anna von Mikecz
    Pages 207-217

  17. Back Matter

    Pages 219-220
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Keywords

About this book

This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects.

Editors and Affiliations

  • Biosystems Group, Biotechnology Center, Silesian University of Technology, Gliwice, Poland

    Ronald Hancock

Bibliographic Information

  • Book Title: The Nucleus

  • Editors: Ronald Hancock

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0763-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0762-6Published: 18 July 2020

  • Softcover ISBN: 978-1-0716-0765-7Published: 18 July 2021

  • eBook ISBN: 978-1-0716-0763-3Published: 17 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 220

  • Number of Illustrations: 12 b/w illustrations, 30 illustrations in colour

  • Topics: Cell Biology, Biochemistry, general

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