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Zika Virus

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2142)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Zika Virus: A Brief History and Review of Its Pathogenesis Rediscovered

    • Joel N. Maslow, Christine C. Roberts
    Pages 1-8
  3. Evaluating Zika Virus Pathogenesis in Immunocompromised Mice

    • Bryan D. Griffin, David Safronetz, Darwyn Kobasa
    Pages 23-40
  4. A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies

    • Kristina Dimitrova, Emelissa J. Mendoza, Nicole Mueller, Heidi Wood
    Pages 59-71
  5. Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation

    • Raquel das Neves Almeida, Heloisa Antoniella Braz-de-Melo, Rafael Corrêa, Kelly Grace Magalhães
    Pages 81-92
  6. Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells

    • Young Chan Kim, Nallely Garcia-Larragoiti, Cesar Lopez-Camacho, Martha Eva Viveros-Sandoval, Arturo Reyes-Sandoval
    Pages 93-102
  7. Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA

    • Young Chan Kim, Maud Dumoux, Raymond J. Owens, Arturo Reyes-Sandoval
    Pages 103-112
  8. Zika Virus Peptide ELISA (ZIKV-NS2B-Concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection

    • Nischay Mishra, Riddhi Thakkar, James Ng, W. Ian Lipkin
    Pages 113-122
  9. Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology

    • Sören Hansen, Oumar Faye, Sabri S. Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye et al.
    Pages 123-136
  10. Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP)

    • Laura E. Lamb, Sarah N. Bartolone, Michael B. Chancellor
    Pages 137-146
  11. Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus

    • Benjamin Lopez-Jimena, Mohammed Bakheit, Michaël Bekaert, Graham Harold, Sieghard Frischmann, Cheikh Fall et al.
    Pages 147-164
  12. Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA

    • Cheikh Tidiane Diagne, Martin Faye, Benjamin Lopez-Jimena, Ahmed Abd El Wahed, Cheikh Loucoubar, Cheikh Fall et al.
    Pages 165-179
  13. A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response

    • Ivan Trus, Stewart Walker, Maria Fuchs, Daniel Udenze, Volker Gerdts, Uladzimir Karniychuk
    Pages 181-195
  14. Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes

    • Nicole Haese, Alec J. Hirsch, Daniel N. Streblow
    Pages 197-213
  15. Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain

    • Daniel Limonta, William Branton, Cheung Pang Wong, Leina Saito, Christopher Power, Tom C. Hobman
    Pages 251-259

About this book

This volume discusses the latest protocols used by scientists to understand ZIKV and how to mitigate its presence. These studies will benefit in the detection, diagnosis, management, treatment, and prevention of this infection. The chapters in this book cover techniques in virus isolation, purification, and ways to titrate the virus; recent advancements made in the development of modified molecular techniques; and a discussion on Zika virus itself to help readers better understand this pathogen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic. 


Reviews

“This book is written for leading scientists in the field of virology and specifically on ZIKV. In my opinion, students, researchers, and scientists in the field of virology and infectious diseases, specifically those interested in Zika virus and mitigating its presence, will find this book extremely useful. The book meets the needs of the intended audience as it provides useful information … .” (Omer Iqbal, Doody's Book Reviews, December 4, 2020)

Editors and Affiliations

  • CHU de Québec - Université Laval, Centre de Recherche en Infectiologie, Québec, Canada

    Gary Kobinger

  • CHU de Québec - Université Laval, Québec, Canada

    Trina Racine

Bibliographic Information

  • Book Title: Zika Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Gary Kobinger, Trina Racine

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0581-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0580-6Published: 05 May 2020

  • Softcover ISBN: 978-1-0716-0583-7Published: 05 May 2021

  • eBook ISBN: 978-1-0716-0581-3Published: 04 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 290

  • Number of Illustrations: 15 b/w illustrations, 42 illustrations in colour

  • Topics: Virology, Infectious Diseases

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access