Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2122)
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Table of contents (21 protocols)
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Front Matter
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Mutagenesis and Genetic Screens
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Microscopic Imaging and Phenotypic Analysis of Arabidopsis Embryos
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Expression Analysis and Transcriptomics
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Protein Interaction Studies
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Embryogenesis in Other Model and Non-model Species
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Front Matter
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Keywords
About this book
This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
The chapter “Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
Bibliographic Information
Book Title: Plant Embryogenesis
Book Subtitle: Methods and Protocols
Editors: Martin Bayer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0342-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0341-3Published: 24 January 2020
Softcover ISBN: 978-1-0716-0344-4Published: 24 January 2021
eBook ISBN: 978-1-0716-0342-0Published: 23 January 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 298
Number of Illustrations: 14 b/w illustrations, 84 illustrations in colour
Topics: Plant Sciences, Embryology