Stem Cell Transcriptional Networks
Methods and Protocols
Editors: Kidder, Benjamin (Ed.)
- includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.
- Table of contents (19 chapters)
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Epitope Tagging ChIP-Seq of DNA Binding Proteins Using CETCh-Seq
Pages 3-34
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User-Friendly and Interactive Analysis of ChIP-Seq Data Using EaSeq
Pages 35-63
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Evaluation of 3D Chromatin Interactions Using Hi-C
Pages 65-78
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MSTD for Detecting Topological Domains from 3D Genomic Maps
Pages 79-92
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Creating 2D Occupancy Plots Using plot2DO
Pages 93-108
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Table of contents (19 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Stem Cell Transcriptional Networks
- Book Subtitle
- Methods and Protocols
- Editors
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- Benjamin Kidder
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2117
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0301-7
- DOI
- 10.1007/978-1-0716-0301-7
- Hardcover ISBN
- 978-1-0716-0300-0
- Softcover ISBN
- 978-1-0716-0303-1
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XI, 308
- Number of Illustrations
- 8 b/w illustrations, 67 illustrations in colour
- Topics
