Skip to main content
SpringerLink
Log in
Book cover

RNA Chaperones

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Tilman Heise

    1. Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, University Hospital of Regensburg, Regensburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2106)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones

    • Hyeong Jin Kim, Steven Chaulk, David Arthur, Ross A. Edwards, J. N. Mark Glover
    Pages 1-18

  3. Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy

    • Subrata Panja, Ewelina M. MaÅ‚ecka, Andrew Santiago-Frangos, Sarah A. Woodson
    Pages 19-39

  4. Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity

    • Pilar Menendez-Gil, Carlos J. Caballero, Cristina Solano, Alejandro Toledo-Arana
    Pages 41-58Open Access

  5. Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding

    • Micah J. McCauley, Ioulia Rouzina, Mark C. Williams
    Pages 59-88

  6. Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein’s RNA Chaperone Activity

    • Tobias Schmidt, Susann Friedrich, Ralph P. Golbik, Sven-Erik Behrens
    Pages 89-106

  7. Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function

    • Jennifer Porat, Mark A. Bayfield
    Pages 107-120

  8. Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La

    • Gunhild Sommer, Christina Sendlmeier, Tilman Heise
    Pages 121-136

  9. Kinetic and Thermodynamic Analyses of RNA–Protein Interactions

    • Ryo Amano, Taiichi Sakamoto
    Pages 137-150

  10. Detection of MicroRNAs Released from Argonautes

    • Kyung-Won Min, J. Grayson Evans, Erick C. Won, Je-Hyun Yoon
    Pages 151-159

  11. Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE Chemical Probing

    • W.-Matthias Leeder, H. Ulrich Göringer
    Pages 161-178

  12. RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing

    • Susann Friedrich, Tobias Schmidt, Sven-Erik Behrens
    Pages 179-192

  13. In Vivo RNA Chemical Footprinting Analysis in Archaea

    • Robert Knüppel, Martin Fenk, Michael Jüttner, Sébastien Ferreira-Cerca
    Pages 193-208

  14. RNA Structure Analysis by Chemical Probing with DMS and CMCT

    • José M. Andrade, Ricardo F. dos Santos, Cecília M. Arraiano
    Pages 209-223

  15. Disordered RNA-Binding Region Prediction with DisoRDPbind

    • Christopher J. Oldfield, Zhenling Peng, Lukasz Kurgan
    Pages 225-239

  16. Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci

    • Shiqi Mao, Yachen Ying, Xiaotian Wu, Antony K. Chen
    Pages 241-252

  17. Site-Specific Dual-Color Labeling of Long RNAs

    • Meng Zhao, Richard Börner, Roland K. O. Sigel, Eva Freisinger
    Pages 253-270

  18. Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding

    • Heesoo Uhm, Sungchul Hohng
    Pages 271-282

  19. Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy

    • M. Nabuan Naufer, Mark C. Williams
    Pages 283-297

  20. Isolation and Analysis of Bacterial Ribosomes Through Sucrose Gradient Ultracentrifugation

    • Ricardo F. dos Santos, Cátia Bárria, Cecília M. Arraiano, José M. Andrade
    Pages 299-310
Back to top

Keywords

About this book

This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones.

Chapter 3 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, University Hospital of Regensburg, Regensburg, Germany

    Tilman Heise

Bibliographic Information

  • Book Title: RNA Chaperones

  • Book Subtitle: Methods and Protocols

  • Editors: Tilman Heise

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0231-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0230-0Published: 31 December 2019

  • Softcover ISBN: 978-1-0716-0233-1Published: 31 December 2020

  • eBook ISBN: 978-1-0716-0231-7Published: 30 December 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 314

  • Number of Illustrations: 8 b/w illustrations, 70 illustrations in colour

  • Topics: Human Genetics, Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation