Overview
- Highlights fundamental aspects of nucleic acids enzymes
- Addresses methods and applications
- Discusses challenges in and prospects for nucleic acid enzyme engineering
Part of the book series: Advances in Biochemical Engineering/Biotechnology (ABE, volume 170)
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Table of contents (6 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This volume reviews numerous reaction mechanisms and applications of nucleic acids with catalytic activity. Written by an interdisciplinary team of authors, it provides an essential overview of these acids’ fundamental aspects, while also addressing associated methodologies such as nucleic acid enzyme engineering, peroxidase-mimicking DNAzymes and Aptazymes.
After the discovery of natural ribozymes – RNA molecules that mediate the cleavage and formation of phosphodiester bonds and the formation of peptide bonds – numerous artificial ribozymes with altered catalytic activities were produced by in vitro and in vivo selection. Unlike ribozymes, DNAzymes do not occur in nature. Although the catalytic activity of nucleic acid enzymes is usually much slower than that of proteins, nucleic acid enzymes with comparable catalytic activity have been obtained using stringent selection processes. The key advantages of these enzymes: they are e.g. smaller, easier to produce and purify than proteins, and can withstand denaturation, e.g. by heat.
Over the last few years, the number of publications on the applications of enzymatic nucleic acids has grown steadily. Summarizing the fundamentals and applications of these acids, this book will not only be an excellent resource for experts in the field but will also guide young researchers just starting out in this significant area.
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: Catalytically Active Nucleic Acids
Editors: Harald Seitz, Frank Stahl, Johanna-Gabriela Walter
Series Title: Advances in Biochemical Engineering/Biotechnology
DOI: https://doi.org/10.1007/978-3-030-29646-9
Publisher: Springer Cham
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Nature Switzerland AG 2020
Hardcover ISBN: 978-3-030-29645-2Published: 11 October 2019
Softcover ISBN: 978-3-030-29648-3Published: 12 October 2020
eBook ISBN: 978-3-030-29646-9Published: 30 September 2019
Series ISSN: 0724-6145
Series E-ISSN: 1616-8542
Edition Number: 1
Number of Pages: VII, 123
Number of Illustrations: 26 b/w illustrations, 12 illustrations in colour
Topics: Biochemical Engineering, Nucleic Acid Chemistry, Enzymology