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Mass Cytometry

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Helen M. McGuire

    1. Discipline of Pathology, The University of Sydney, Camperdown, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Thomas M. Ashhurst

    1. Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1989)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. Setting Up a Facility

    1. Front Matter

      Pages 1-1
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    2. Setting Up Mass Cytometry in a Shared Resource Lab Environment

      • Sarah Warth, Désirée Kunkel
      Pages 3-11

    3. Acquisition, Processing, and Quality Control of Mass Cytometry Data

      • Brian H. Lee, Adeeb H. Rahman
      Pages 13-31

  3. Panel Design and Reagent Preparation

    1. Front Matter

      Pages 33-33
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    2. Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating

      • Amelia Au-Yeung, Chikara Takahashi, W. Rodney Mathews, William E. O’Gorman
      Pages 35-45

    3. Method for Tagging Antibodies with Metals for Mass Cytometry Experiments

      • Stephen Gregory Chang, Cynthia J. Guidos
      Pages 47-54

    4. Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis

      • Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan et al.
      Pages 55-81

    5. Titration of Mass Cytometry Reagents

      • Caryn van Vreden, Paula Niewold, Helen M. McGuire, Barbara Fazekas de St. Groth
      Pages 83-92

    6. Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry

      • Axel Ronald Schulz, Henrik E. Mei
      Pages 93-108

  4. Sample Preparation

    1. Front Matter

      Pages 109-109
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    2. Automated Cell Processing for Mass Cytometry Experiments

      • Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, Petter Brodin
      Pages 111-123

    3. Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry

      • Lisa E. Wagar
      Pages 125-135

  5. Specific Applications

    1. Front Matter

      Pages 137-137
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    2. Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry

      • Diana Shinko, Thomas M. Ashhurst, Helen M. McGuire, Kellie A. Charles
      Pages 139-146

    3. Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry

      • Yannick Simoni, Michael Fehlings, Evan W. Newell
      Pages 147-158

    4. Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometry

      • Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, Nicholas J. C. King
      Pages 159-192

    5. Mass Cytometric Cell Cycle Analysis

      • Gregory K. Behbehani
      Pages 193-215

    6. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry

      • Mikael Roussel, Todd Bartkowiak, Jonathan M. Irish
      Pages 217-226

    7. Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry

      • Andrew J. Mitchell, Angela Ivask, Yi Ju
      Pages 227-241
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Keywords

About this book

This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Discipline of Pathology, The University of Sydney, Camperdown, Australia

    Helen M. McGuire

  • Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, Australia

    Thomas M. Ashhurst

Bibliographic Information

  • Book Title: Mass Cytometry

  • Book Subtitle: Methods and Protocols

  • Editors: Helen M. McGuire, Thomas M. Ashhurst

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9454-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9453-3Published: 13 May 2019

  • eBook ISBN: 978-1-4939-9454-0Published: 10 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 338

  • Number of Illustrations: 13 b/w illustrations, 70 illustrations in colour

  • Topics: Cell Biology, Mass Spectrometry, Antibodies

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