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Microcalorimetry of Biological Molecules

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Eric Ennifar

    1. Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1964)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Differential Scanning Calorimetry

    1. Front Matter

      Pages 1-1
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    2. The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions

      • Marie-Lise Jobin, Isabel D. Alves
      Pages 3-15

    3. Protocols of IATC, DSC, and PPC: The Multistate Structural Transition of Cytochrome c

      • Shigeyoshi Nakamura, Shun-ichi Kidokoro
      Pages 17-32

    4. Value of DSC in Characterization and Optimization of Protein Stability

      • Katherine Bowers, Natalia Markova
      Pages 33-44

    5. Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC)

      • Philipp O. Tsvetkov, François Devred
      Pages 45-57

  3. Isothermal Titration Calorimetry

    1. Front Matter

      Pages 59-59
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    2. Intrinsic Thermodynamics of Protein-Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design

      • Vaida Paketurytė, Asta Zubrienė, John E. Ladbury, Daumantas Matulis
      Pages 61-74

    3. Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions

      • Christopher P. Jones, Grzegorz Piszczek, Adrian R. Ferré-D’Amaré
      Pages 75-87

    4. ITC Studies of Ribosome/Antibiotics Interactions

      • Emma Schenckbecher, Benoît Meyer, Eric Ennifar
      Pages 89-98

    5. High-Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry

      • Juan Ramirez, Yves Nominé
      Pages 99-117

    6. ITC Measurement for High-Affinity Aptamers Binding to Their Target Proteins

      • Ryo Amano, Tomohisa Furukawa, Taiichi Sakamoto
      Pages 119-128

    7. Thermodynamics of Molecular Machines Using Incremental ITC

      • Benoît Meyer, Cyrielle da Veiga, Philippe Dumas, Eric Ennifar
      Pages 129-140

    8. Measuring the Metabolic Activity of Mature Mycobacterial Biofilms Using Isothermal Microcalorimetry

      • Anna Solokhina, Gernot Bonkat, Olivier Braissant
      Pages 141-149

    9. Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC)

      • Philipp O. Tsvetkov, Romain La Rocca, Soazig Malesinski, François Devred
      Pages 151-165

  4. Advanced Data Processing and Kinetics

    1. Front Matter

      Pages 167-167
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    2. Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC

      • Ibrahim Q. Saeed, Niklaas J. Buurma
      Pages 169-183

    3. Tinkering with Binding Polynomials in Isothermal Titration Calorimetry

      • Rafael Claveria-Gimeno, Sonia Vega, Olga Abian, Adrian Velazquez-Campoy
      Pages 185-213

    4. The Use of ITC and the Software AFFINImeter for the Quantification of the Anticoagulant Pentasaccharide in Low Molecular Weight Heparin

      • Eva Muñoz, Juan Sabín
      Pages 215-223

    5. Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by Using KinITC in AFFINImeter

      • Eva Muñoz, Juan Sabín, Javier Rial, Daniel Pérez, Eric Ennifar, Philippe Dumas et al.
      Pages 225-239
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Keywords

About this book

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Editors and Affiliations

  • Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

    Eric Ennifar

Bibliographic Information

  • Book Title: Microcalorimetry of Biological Molecules

  • Book Subtitle: Methods and Protocols

  • Editors: Eric Ennifar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9179-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9178-5Published: 01 April 2019

  • eBook ISBN: 978-1-4939-9179-2Published: 30 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 268

  • Number of Illustrations: 20 b/w illustrations, 40 illustrations in colour

  • Topics: Biochemistry, general

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