Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2019

Collagen

Methods and Protocols

Editors:

  1. Irit Sagi

    1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Nikolaos A. Afratis

    1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1944)

Sections

Buy it now

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. In Vitro Models for Collagen Characterization

    1. Front Matter

      Pages 1-1
      PDF

    2. Cloning and Mutagenesis Strategies for Large Collagens

      • Olivier Bornert, Alexander Nyström
      Pages 3-15

    4. Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

      • Ignacio Sallent, Héctor Capella-Monsonís, Dimitrios I. Zeugolis
      Pages 23-38

    5. A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

      • Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang
      Pages 39-54

  3. Proteomics Analysis of Collagen

    1. Front Matter

      Pages 55-55
      PDF

    3. Measurement of Collagen Cross-Links from Tissue Samples by Mass Spectrometry

      • Amar Joshi, Amna Zahoor, Alberto Buson
      Pages 79-93

    4. Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes

      • Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, Matthew D. Shoulders
      Pages 95-114

    5. Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics

      • Elizabeta Madzharova, Fabio Sabino, Ulrich auf dem Keller
      Pages 115-126

  4. Imaging Approaches for Collagen

    1. Front Matter

      Pages 127-127
      PDF

    4. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

      • Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, Jean-Marc Allain et al.
      Pages 145-155

  5. Collagen in Health and Disease

    1. Front Matter

      Pages 167-167
      PDF

    2. Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans

      • Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, Collin Y. Ewald
      Pages 169-188

    3. Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis

      • Efrat Kessler, Eyal Hassoun
      Pages 189-201
Back to top

About this book

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.
Back to top

Keywords

Back to top

Editors and Affiliations

  • Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Irit Sagi, Nikolaos A. Afratis

Back to top

Bibliographic Information

  • Book Title: Collagen

  • Book Subtitle: Methods and Protocols

  • Editors: Irit Sagi, Nikolaos A. Afratis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9095-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9094-8Published: 07 March 2019

  • eBook ISBN: 978-1-4939-9095-5Published: 06 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 254

  • Number of Illustrations: 16 b/w illustrations, 44 illustrations in colour

  • Topics: Protein Science, Cell Biology

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation