Methods in Molecular Biology

Calpain

Methods and Protocols

Editors: Messer, Jeannette S. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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イーブック ¥19,093
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8988-1
  • ウォーターマーク付、 DRMフリー
  • ファイル形式: EPUB, PDF
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ハードカバー ¥23,867
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8987-4
  • 個人のお客様には、世界中どこでも配送料無料でお届けします。
  • Usually dispatched within 3 to 5 business days.
この書籍について

This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases.


Table of contents (20 chapters)

Table of contents (20 chapters)
  • Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin Using Anion-Exchange Chromatography

    Biswas, Ashim Kumar (et al.)

    Pages 3-11

  • Bacterial Expression and Purification of Calpains

    McCartney, Christian-Scott E. (et al.)

    Pages 13-27

  • Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity

    Biswas, Ashim Kumar (et al.)

    Pages 31-38

  • FRET-Based Assays to Determine Calpain Activity

    McCartney, Christian-Scott E. (et al.)

    Pages 39-55

  • In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer

    Lostal, William (et al.)

    Pages 57-66

書籍の購入

イーブック ¥19,093
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8988-1
  • ウォーターマーク付、 DRMフリー
  • ファイル形式: EPUB, PDF
  • どの電子書籍リーダーからでもすぐにお読みいただけます。
  • ご購入後、すぐにダウンロードしていただけます。
ハードカバー ¥23,867
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8987-4
  • 個人のお客様には、世界中どこでも配送料無料でお届けします。
  • Usually dispatched within 3 to 5 business days.
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書誌情報

Bibliographic Information
Book Title
Calpain
Book Subtitle
Methods and Protocols
Editors
  • Jeannette S. Messer
Series Title
Methods in Molecular Biology
Series Volume
1915
Copyright
2019
Publisher
Springer-Verlag New York
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
イーブック ISBN
978-1-4939-8988-1
DOI
10.1007/978-1-4939-8988-1
ハードカバー ISBN
978-1-4939-8987-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 276
Number of Illustrations
29 b/w illustrations, 37 illustrations in colour
Topics