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Protein Gel Detection and Imaging

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Biji T. Kurien

    1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. R. Hal Scofield

    1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1853)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Protein Stains and Applications

    • Pazhani Sundaram
    Pages 1-14

  3. The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS–Polyacrylamide Electrophoresis Gel

    • J. P. Dean Goldring
    Pages 15-18

  4. Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE

    • Gaurav Kumar
    Pages 19-25

  5. Coomassie Brilliant Blue Staining of Polyacrylamide Gels

    • Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, Michael Bachmann
    Pages 27-30

  6. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue

    • Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, Tian-yun Wang
    Pages 31-35

  7. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue

    • Biji T. Kurien, R. Hal Scofield
    Pages 37-45

  8. Silver Staining Techniques of Polyacrylamide Gels

    • Nicole Berndt, Ralf Bergmann, Claudia Arndt, Stefanie Koristka, Michael Bachmann
    Pages 47-52

  9. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

    • Sun-Young Hwang, Jung-Kap Choi
    Pages 53-64

  10. Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain

    • Sun-Young Hwang, Xu Wang, Jung-Kap Choi
    Pages 65-73

  11. Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins

    • Jennie R. Lill, Victor J. Nesatyy
    Pages 75-86

  12. Fluorescent Staining of Gels

    • Engelbert Buxbaum
    Pages 87-94

  13. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis

    • Jayanta K. Pal, Sunil K. Berwal, Rupali N. Soni
    Pages 95-103

  14. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE

    • Na Na, Jin Ouyang
    Pages 105-114

  15. Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain

    • Padmaja Mehta-D’souza
    Pages 115-119

  16. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain

    • Biji T. Kurien, Yaser Dorri, R. Hal Scofield
    Pages 121-131

  17. Detection of Multiple Enzymes in Fermentation Broth Using Single PAGE Analysis

    • K. Divakar, J. Deepa Arul Priya, G. Panneer Selvam, M. Suryia Prabha, Ashwin Kannan, G. Nandhini Devi et al.
    Pages 133-138

  18. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis

    • Han-Min Chen
    Pages 139-147

  19. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

    • Jelena Bogdanović Pristov, Ivan Spasojević
    Pages 149-158

  20. Ten Minute Stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black

    • J. P. Dean Goldring, Robert G. E. Krause
    Pages 159-163
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Keywords

About this book

This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.



Editors and Affiliations

  • Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    Biji T. Kurien, R. Hal Scofield

Bibliographic Information

  • Book Title: Protein Gel Detection and Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Biji T. Kurien, R. Hal Scofield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8745-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8744-3Published: 10 August 2018

  • Softcover ISBN: 978-1-4939-9381-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8745-0Published: 10 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 289

  • Number of Illustrations: 4 b/w illustrations, 35 illustrations in colour

  • Topics: Protein Science

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