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Methods in Molecular Biology

The CRAC Channel

Methods and Protocols

Editors: Penna, Aubin, Constantin, Bruno (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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書籍の購入

イーブック ¥17,389
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8704-7
  • ウォーターマーク付、 DRMフリー
  • ファイル形式: PDF, EPUB
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  • ご購入後、すぐにダウンロードしていただけます。
ハードカバー ¥21,737
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8702-3
  • 個人のお客様には、世界中どこでも配送料無料でお届けします。
  • Usually dispatched within 3 to 5 business days.
この書籍について

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learning more about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.

Table of contents (16 chapters)

  • Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells

    Zhang, Yiming (et al.)

    Pages 1-16

    Preview Buy Chapter ¥5,399
  • Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai-Overexpressing HEK-293 Cells

    Zhang, Shuce (et al.)

    Pages 17-39

    Preview Buy Chapter ¥5,399
  • Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging

    Wu, Hsiang-en (et al.)

    Pages 41-53

  • Mn2+ Quenching Assay for Store-Operated Calcium Entry

    Pan, Zui (et al.)

    Pages 55-62

  • Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells Using Fluo-4 and Confocal Live-Cell Imaging

    Lu, Fujian (et al.)

    Pages 63-68

書籍の購入

イーブック ¥17,389
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8704-7
  • ウォーターマーク付、 DRMフリー
  • ファイル形式: PDF, EPUB
  • どの電子書籍リーダーからでもすぐにお読みいただけます。
  • ご購入後、すぐにダウンロードしていただけます。
ハードカバー ¥21,737
価格の適用国: Japan (日本円価格は個人のお客様のみ有効) (小計)
  • ISBN 978-1-4939-8702-3
  • 個人のお客様には、世界中どこでも配送料無料でお届けします。
  • Usually dispatched within 3 to 5 business days.
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書誌情報

Bibliographic Information
Book Title
The CRAC Channel
Book Subtitle
Methods and Protocols
Editors
  • Aubin Penna
  • Bruno Constantin
Series Title
Methods in Molecular Biology
Series Volume
1843
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
イーブック ISBN
978-1-4939-8704-7
DOI
10.1007/978-1-4939-8704-7
ハードカバー ISBN
978-1-4939-8702-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 204
Number of Illustrations
15 b/w illustrations, 26 illustrations in colour
Topics