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Amyloid Proteins

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Einar M. Sigurdsson

    1. Department of Neuroscience and Physiology, New York University School of Medicine, New York University Langone Health, New York, USA

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  2. Miguel Calero

    1. Chronic Disease Programme-CROSADIS CIBERNED, Queen Sofia Foundation, Alzheimer Center CIEN Foundation, Instituto de Salud Carlos III, Madrid, Spain

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  3. María Gasset

    1. Institute of Physical Chemistry “Rocasolano”, Spanish National Research Council (CSIC), Madrid, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1779)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. In Vitro Models and Assays

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Preparation of Pure Populations of Amyloid β-Protein Oligomers of Defined Size

      • Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, David B. Teplow
      Pages 3-12

    3. Preparation of a Well-Defined and Stable β-Barrel Pore-Forming Aβ42 Oligomer

      • Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, Natàlia Carulla
      Pages 13-22

    4. Unveiling Brain Aβ Heterogeneity Through Targeted Proteomic Analysis

      • Agueda Rostagno, Thomas A. Neubert, Jorge Ghiso
      Pages 23-43

    5. Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments

      • Serene W. Chen, Nunilo Cremades
      Pages 45-60

    6. Generation and Characterization of Stable α-Synuclein Oligomers

      • Leire Almandoz-Gil, Martin Ingelsson, Joakim Bergström
      Pages 61-71

    7. In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity

      • Istvan Horvath, Sandra Rocha, Pernilla Wittung-Stafshede
      Pages 73-83

    8. Preparation of Tau Oligomers After the Protein Extraction from Bacteria and Brain Cortices

      • Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, Ottavio Arancio
      Pages 85-97

    9. Purification and Characterization of Low-n Tau Oligomers

      • Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, Eckhard Mandelkow
      Pages 99-111

    10. Preparation and Characterization of Tau Oligomer Strains

      • Urmi Sengupta, Mariana Carretero-Murillo, Rakez Kayed
      Pages 113-146

    11. Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein, and Tau Under Native Conditions

      • Alexander Sandberg, Sofie Nyström
      Pages 147-166

    12. Preparation of Amyloidogenic Aggregates from EF-Hand β-Parvalbumin and S100 Proteins

      • Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset, Cláudio M. Gomes
      Pages 167-179

    13. Kinetic Analysis of Amyloid Formation

      • Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles
      Pages 181-196

    14. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays

      • Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, Maria Gasset
      Pages 197-207

    15. Noninvasive Structural Analysis of Intermediate Species During Fibrillation: An Application of Small-Angle X-Ray Scattering

      • Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, Bente Vestergaard
      Pages 209-239

    16. Analysis of Covalent Modifications of Amyloidogenic Proteins Using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation

      • Elizaveta Katorcha, Ilia V. Baskakov
      Pages 241-255

    17. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using the Real-Time Quaking-Induced Conversion

      • Matthias Schmitz, Niccolò Candelise, Franc Llorens, Inga Zerr
      Pages 257-263

  3. Cell Culture Models and Assays

    1. Front Matter

      Pages 265-265
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    2. Bacterial Amyloids

      • Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, Matthew R. Chapman
      Pages 267-288
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Keywords

About this book

This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety  of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.


Editors and Affiliations

  • Department of Neuroscience and Physiology, New York University School of Medicine, New York University Langone Health, New York, USA

    Einar M. Sigurdsson

  • Chronic Disease Programme-CROSADIS CIBERNED, Queen Sofia Foundation, Alzheimer Center CIEN Foundation, Instituto de Salud Carlos III, Madrid, Spain

    Miguel Calero

  • Institute of Physical Chemistry “Rocasolano”, Spanish National Research Council (CSIC), Madrid, Spain

    María Gasset

Bibliographic Information

  • Book Title: Amyloid Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Einar M. Sigurdsson, Miguel Calero, María Gasset

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7816-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7815-1Published: 11 June 2018

  • Softcover ISBN: 978-1-4939-9293-5Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7816-8Published: 09 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVII, 547

  • Number of Illustrations: 34 b/w illustrations, 65 illustrations in colour

  • Topics: Protein Science

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