Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2407)
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Table of contents (28 protocols)
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Front Matter
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Cell Lines with Minimal but Inducible Proviral Expression
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Front Matter
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In Vitro and Ex Vivo Primary Cell Models of Minimal but Inducible Proviral Expression
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Front Matter
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In Vitro and Ex Vivo Tissue-Derived Models of Reversible Proviral Latency
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Front Matter
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About this book
This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.
Editors and Affiliations
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School of Medicine and Human Immuno-Virology (H.I.V.) Group, San Raffaele University and Scientific Institute, Milano, Italy
Guido Poli
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Viral Pathogenesis and Biosafety Group, San Raffaele Scientific Institute, Milano, Italy
Elisa Vicenzi
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Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, USA
Fabio Romerio
Bibliographic Information
Book Title: HIV Reservoirs
Book Subtitle: Methods and Protocols
Editors: Guido Poli, Elisa Vicenzi, Fabio Romerio
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1871-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1870-7Published: 06 January 2022
Softcover ISBN: 978-1-0716-1873-8Published: 07 January 2023
eBook ISBN: 978-1-0716-1871-4Published: 05 January 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 451
Number of Illustrations: 61 b/w illustrations
Topics: Infectious Diseases