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  • © 2021

Bifidobacteria

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2278)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Methods for Isolation and Recovery of Bifidobacteria

    • Abelardo Margolles, Lorena Ruiz
    Pages 1-12
  3. Bifidobacterium Transformation

    • Emily C. Hoedt, Roger S. Bongers, Francesca Bottacini, Jan Knol, John MacSharry, Douwe van Sinderen
    Pages 13-19
  4. Isolation of Chromosomal and Plasmid DNA from Bifidobacteria

    • Maria Esteban-Torres, Lorena Ruiz, Rocio Sanchez-Gallardo, Douwe van Sinderen
    Pages 21-29
  5. Site-Directed Mutagenesis of Bifidobacterium Strains

    • Kieran James, Douwe van Sinderen
    Pages 45-60
  6. Protocol to Select Bifidobacteria from Fecal and Environmental Samples

    • Giulia Alessandri, Maria Cristina Ossiprandi, Marco Ventura, Douwe van Sinderen
    Pages 61-70
  7. Phageome Analysis of Bifidobacteria-Rich Samples

    • Brian McDonnell, Eoghan Casey, Christian Milani, Gabriele Andrea Lugli, Alice Viappiani, Jennifer Mahony et al.
    Pages 71-85
  8. Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria

    • Grace Ahern, Douwe van Sinderen, Bo Yang, R. Paul Ross, Catherine Stanton
    Pages 87-100
  9. Model for Murine Gut Colonization by Bifidobacteria

    • Valerio Rossini, Ken Nally
    Pages 131-139
  10. Identification of Bifidobacteria by the Phosphoketolase Assay

    • Monica Modesto, Alice Checcucci, Paola Mattarelli
    Pages 141-148
  11. Determination of Bile Salt Hydrolase Activity in Bifidobacteria

    • Lorena Ruiz, Borja Sánchez, Abelardo Margolles
    Pages 149-155
  12. Resistance of Bifidobacteria Toward Antibiotics

    • Miguel Gueimonde, Silvia Arboleya
    Pages 195-208
  13. In Vitro Assessment of Prebiotic Activity

    • Alberto Amaretti, Stefano Raimondi, Nicola Volpi, Maddalena Rossi
    Pages 209-223
  14. Bifidobacterium Genome Assembly and Methylome Analysis Using Pacbio SMRT Sequencing

    • Francesca Bottacini, Douwe van Sinderen
    Pages 225-232
  15. Back Matter

    Pages 233-239

About this book

This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.


Editors and Affiliations

  • Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

    Douwe van Sinderen

  • Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    Marco Ventura

Bibliographic Information

  • Book Title: Bifidobacteria

  • Book Subtitle: Methods and Protocols

  • Editors: Douwe van Sinderen, Marco Ventura

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1274-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1273-6Published: 03 March 2021

  • Softcover ISBN: 978-1-0716-1276-7Published: 04 March 2022

  • eBook ISBN: 978-1-0716-1274-3Published: 02 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 239

  • Number of Illustrations: 8 b/w illustrations, 18 illustrations in colour

  • Topics: Bacteriology, Gastroenterology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access