PDZ Mediated Interactions

1. Front Matter

   Pages i-xi

   PDF

2. Identification of PDZ Interactions by Yeast Two-Hybrid Technique

   • Monica Castro-Cruz, Marta Monserrat-Gomez, Jean-Paul Borg, Pascale Zimmermann, Eric Bailly

   Pages 1-15

3. Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis

   • Avais M. Daulat, Stéphane Audebert, Mônica Wagner, Luc Camoin, Jean-Paul Borg

   Pages 17-40

4. Identification of PDZ Interactions by Proteomic Peptide Phage Display

   • Susanne Lüchow, Gustav N. Sundell, Ylva Ivarsson

   Pages 41-60

5. A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay

   • Pau Jané, Lionel Chiron, Goran Bich, Gilles Travé, Yves Nominé

   Pages 61-74

6. Study of PDZ–Peptide and PDZ–Lipid Interactions by Surface Plasmon Resonance/BIAcore

   • Pascale Zimmermann, Antonio Luis Egea-Jimenez

   Pages 75-87

7. PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations

   • Célia Caillet-Saguy, Sébastien Brûlé, Nicolas Wolff, Bertrand Raynal

   Pages 89-124

8. Crystallographic Studies of PDZ Domain–Peptide Interactions of the Scribble Polarity Module

   • Janesha C. Maddumage, Bryce Z. Stewart, Patrick O. Humbert, Marc Kvansakul

   Pages 125-135

9. A Fluorescence-Based Assay to Determine PDZ–Ligand Binding Thermodynamics

   • Young Joo Sun, Ernesto J. Fuentes

   Pages 137-148

10. Unveiling the Folding Mechanism of PDZ Domains

    • Candice Gautier, Stefano Gianni

    Pages 149-156

11. Development of Peptide-Based PDZ Domain Inhibitors

    • Dominik J. Essig, Javier R. Balboa, Kristian Strømgaard

    Pages 157-177

12. Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs

    • Márton A. Simon, László Nyitray

    Pages 179-192

13. Chemical Synthesis of PDZ Domains

    • Christin Kossmann, Sana Ma, Louise S. Clemmensen, Kristian Strømgaard

    Pages 193-216

14. Viral PDZ Binding Motifs Influence Cell Behavior Through the Interaction with Cellular Proteins Containing PDZ Domains

    • Carlos Castaño-Rodriguez, Jose M. Honrubia, Javier Gutiérrez-Álvarez, Isabel Sola, Luis Enjuanes

    Pages 217-236

15. Computational Design of PDZ-Peptide Binding

    • Nicolas Panel, Francesco Villa, Vaitea Opuu, David Mignon, Thomas Simonson

    Pages 237-255

16. Mechanoregulation of PDZ Proteins, An Emerging Function

    • Elsa Bazellières, André Le Bivic

    Pages 257-275

17. Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains

    • Laurent Hoffer, Philippe Roche, Xavier Morelli

    Pages 277-289

18. Back Matter

    Pages 291-292

    PDF

This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field. 

• protein-lipid interactions
• two-hybrids in the yeast
• peptide phage display
• super-binder peptides
• PDZ domains

• Centre de Recherche en CancÕrologie de Marseille, Aix-Marseille University, Inserm, CNRS, Institut Paoli-Calmettes, Marseille, France

  Jean-Paul Borg

Policies and ethics