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Detection of Cell Death Mechanisms

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ayesha B. Alvero

    1. Department of Obstetrics and Gynecology, Wayne State University, Detroit, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Gil G. Mor

    1. Department of Obstetrics and Gynecology, Wayne State University, Detroit, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2255)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Determination of Caspase Activation by Western Blot

    • Hussein Chehade, Alexandra Fox, Gil G. Mor, Ayesha B. Alvero
    Pages 1-12

  3. Detection of Unfolded Protein Response by Polymerase Chain Reaction

    • Alexander Paridon, Alexandra Fox, Ayesha B. Alvero
    Pages 13-20

  4. Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway

    • Hussein Chehade, Alexandra Fox, Gil G. Mor, Ayesha B. Alvero
    Pages 21-26

  5. A Triple-Parameter-Based Laboratory-Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells

    • Pradip De, Jennifer Carlson Aske, Nandini Dey
    Pages 27-42

  6. Flow Cytometric Analyses of p53-Mediated Cell Cycle Arrest and Apoptosis in Cancer Cells

    • Nour N. Al Zouabi, Cai M. Roberts, Z. Ping Lin, Elena S. Ratner
    Pages 43-53

  7. A Real-Time, Bioluminescent Apoptosis Assay

    • Kevin R. Kupcho, Andrew L. Niles
    Pages 55-67

  8. Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity

    • Roslyn Tedja, Alexandra Fox, Ayesha B. Alvero
    Pages 69-76

  9. Time- and Dose-Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay

    • Natasha Karassina, Peter Hofsteen, James J. Cali, Jolanta Vidugiriene
    Pages 77-86

  10. Visualization and Quantification of Neutrophil Extracellular Traps

    • Mancy Tong, Vikki M. Abrahams
    Pages 87-95

  11. In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

    • Guillermina Calo, Analia Silvina Trevani, Esteban Grasso, Irene Angelica Keitelman, Rosanna Ramhorst, Claudia Pérez Leirós et al.
    Pages 97-117

  12. Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture

    • Mikhail Chesnokov, Imran Khan, Ilana Chefetz
    Pages 119-134

  13. Visualization of Necroptotic Cell Death through Transmission Electron Microscopy

    • Naresh Golla, Linda J. Hong, Ilana Chefetz
    Pages 135-147

  14. Methodology for Comprehensive Detection of Pyroptosis

    • Yang Feng, Xiaoli Huang
    Pages 149-157

  15. Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death

    • Han-Hsuan Fu, Harry Qui
    Pages 159-169

  16. Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model

    • Kazuki Tatsuno, Patrick Han, Richard Edelson, Douglas Hanlon
    Pages 171-186

  17. A Luminescence Assay to Quantify Cell Viability in Real Time

    • Peter Hofsteen, Natasha Karassina, James J. Cali, Jolanta Vidugiriene
    Pages 187-196

  18. Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis

    • Jill E. Granger, Daniel M. Appledorn
    Pages 197-212

  19. Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes

    • Cai M. Roberts, Elena S. Ratner
    Pages 213-232

  20. Determining Cell Death Pathway and Regulation by Enrichment Analysis

    • Katherine Gurdziel
    Pages 233-239
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Keywords

About this book

This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.


Editors and Affiliations

  • Department of Obstetrics and Gynecology, Wayne State University, Detroit, USA

    Ayesha B. Alvero, Gil G. Mor

Bibliographic Information

  • Book Title: Detection of Cell Death Mechanisms

  • Book Subtitle: Methods and Protocols

  • Editors: Ayesha B. Alvero, Gil G. Mor

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1162-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1161-6Published: 15 December 2020

  • Softcover ISBN: 978-1-0716-1164-7Published: 15 December 2021

  • eBook ISBN: 978-1-0716-1162-3Published: 14 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 265

  • Number of Illustrations: 6 b/w illustrations, 60 illustrations in colour

  • Topics: Apoptosis

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